An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains

Dijken, J.P. van; Bauer, J.; Brambilla, L.; Duboc, P.; Francois, J.M.; Gancedo, C.; Giuseppin, M.L.F.; Heijnen, J.J.; Hoare, M.; Lange, H.C.; Madden, E.A.; Niederberger, P.; Nielsen, J.; Parrou, Jean-Luc; Petit, T.; Porro, D.; Reuss, M.; Van Riel, N; Rizzi, M.; Steensma, H.Y.; Verrips, C.T.; Vindeløv, J.; Pronk, J.T.

An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains

2000

To select a Saccharomyces cerevisiae reference strain amenable to experimental techniques used in (molecular) genetic, physiological and biochemical engineering research, a variety of properties were studied in four diploid, prototrophic laboratory strains. The following parameters were investigated: 1) maximum specific growth rate in shake-flask cultures; 2) biomass yields on glucose during growth on defined media in batch cultures and steady-state chemostat cultures under controlled conditions with respect to pH and dissolved oxygen concentration; 3) the critical specific growth rate above which aerobic fermentation becomes apparent in glucose-limited accelerostat cultures; 4) sporulation and mating efficiency; and 5) transformation efficiency via the lithium-acetate, bicine, and electroporation methods. On the basis of physiological as well as genetic properties, strains from the CEN.PK family were selected as a platform for cell-factory research on the stoichiometry and kinetics of growth and product formation.

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