Callus induction and establishment of suspension culture from seed derived cotyledon of cabbage cv. 864 Green Storm
2004
Bahrun D. | Rashid A. A. | Kadir M. A. | Aziz M. A.
Callus of cabbage (Brassica oleracea var. capitata L.) were initiated from cotyledon explants derived from seeds. Cotyledon explants cultured on medium containing 8 and 10 mgL-¹ 2,4-D resulted in high intensity of callus formation with 75-100% of the explant surface covered with callus. The callus produced in these treatments was friable, whitish and translucent. On medium without 2,4-D, no callus formation was observed. Upon transfer of the friable callus to liquid MS medium containing 1 mgL-¹ 2,4-D, rounded cells with dense cytoplasm (embryogenic) were obtained.
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