Sensitivity and specificity of polyclonal antisera for determination of Clavibacter michiganensis subsp. sepedonicus and their use by the slide agglutination method
1998
Kokoskova, B. | Pankova, I. (Vyzkumny Ustav Rostlinne Vyroby, Prague-Ruzyne (Czech Republic). Odbor Rostlinolekarstvi)
Two polyclonal antisera for determination of Clavibacter michiganenisis subsp. sepedonicus, the causal agent of potato bacterial ring rot, were prepared. The titres of these antisera were 1 : 128 for antiserum CS 6 and 1 : 64 for antiserum CS 7. Antiserum CS 7 with a lower titre was used for slide agglutination after absorption with cross reacting bacteria (Pseudomonas fluorescens, Ralstonia solanacearum, Clavibacter michiganensis subsp. isidiosus). Antiserum specificity was verified with a group of Cms bacterial strains. The antiserum CS 6 with a higher titre was used for preparation of IgG and IgG-AP that will be used to detect Cms in samples of potato tubers by the ELISA method.
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