Rapid measurement and evaluation of the effect of drying conditions on harpagoside content in Harpagophytum procumbens (devil's claw) root
2005
Joubert, E. | Manley, M. | Gray, B.R. | Schulz, H.
The effect of drying conditions on harpagoside (HS) retention, as well as the use of near-infrared spectroscopy (NIRS) for rapid quantification of the iridoids, HS, and 8-rho-coumaroyl harpagide (8rhoCHG) and moisture, in dried Harpagophytum procumbens (devil's claw) root was investigated. HS retention was significantly (P < 0.05) lower in sun-dried samples as compared to tunnel-dried (60 degrees C, 30% relative humidity) and freeze-dried samples. The best retention of HS was obtained at 50 degrees C when evaluating tunnel drying at dry bulb temperatures of 40, 50, and 60 degrees C and 30% relative humidity. NIRS can effectively predict moisture content with a standard error of prediction (SEP) and correlation coefficient (r) of 0.24% and 0.99, respectively. The HS and 8rhoCHG NIRS calibration models established for both iridoid glucosides can be used for screening purposes to get a semiquantitative classification of devil's claw roots (for HS: SEP = 0.236%, r = 0.64; for 8rhoCHG: SEP = 0.048%, r = 0.73).
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