Relationships of PPAR gamma and PPAR gamma 2 mRNA levels to obesity, diabetes and hyperinsulinaemia in rhesus monkeys

Hotta, K.; Gustafson, T.A.; Yoshioka, S.; Ortmeyer, H.K.; Bodkin, N.L.; Hansen, B.C.

Relationships of PPAR gamma and PPAR gamma 2 mRNA levels to obesity, diabetes and hyperinsulinaemia in rhesus monkeys

1998

OBJECTIVE: To examine the expression of peroxisome proliferator-activated receptor gamma (PPAR gamma) together with CCAAT/enhancer binding protein alpha (C/EBP alpha), lipoprotein lipase (LPL) and glucose transporter (GLUT4) mRNA in adipose tissue of rhesus monkeys in relation to obesity. DESIGN: Cloning of the PPAR gamma 1 and gamma 2 cDNAs and analysis of PPAR gamma, C/EBP alpha, LPL and GLUT4 mRNA levels in the adipose tissue of lean and obese monkeys. SUBJECTS: 28 rhesus monkeys (Macaca mulatta) with a wide range of body weights (9.2-22.6 kg) and with or without type 2 diabetes. MEASUREMENTS: Sequence of PPAR gamma 1 and gamma 2. Tissue distribution of PPAR gamma 1 and gamma 2. The mRNA levels of PPAR gamma, C/EBP alpha, LPL and GLUT4 in adipose tissue. The ratio of PPAR gamma 2 mRNA to total PPAR gamma mRNA. RESULTS: The monkey PPAR gamma 2 protein showed 99% identity with the human protein. PPAR gamma 1 mRNA was shown to be expressed in various tissues and most abundantly in adipose tissue. PPAR gamma 2 existed mainly in adipose tissue. A significant correlation between the ratio of PPAR gamma 2 mRNA to total PPAR gamma mRNA and obesity was observed, whereas total PPAR gamma mRNA levels showed no significant relationships to obesity. There was also a significant relationship between the ratio of PPAR gamma 2 mRNA to total PPAR gamma mRNA and fasting plasma insulin concentration. The mRNA levels of C/EBP alpha, LPL and GLUT4 were highly correlated to that of total PPAR gamma mRNA. They were also significantly correlated to the mRNA levels of PPAR gamma 1 and PPAR gamma 2. CONCLUSIONS: The ratio of PPAR gamma 2 mRNA to total PPAR gamma mRNA is related to obesity in the rhesus monkey and mRNA expression of PPAR gamma 1, PPAR gamma 2, C/EBP alpha, LPL and GLUT4 appear to be coordinated in vivo.

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Mots clés AGROVOC

adipose tissue adipose tissue amino acid sequences animals binding proteins chemistry complementary dna diabetes diabetes mellitus dna gene expression genetics glucose histochemistry humans lipoprotein lipase messenger rna metabolism mice obesity obesity receptors receptors rna transcription factors

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Publié dans

International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity

Volume 22 Numéro 10 Pagination 1000 - 1010 ISSN 0307-0565

Editeur

Elsevier Ltd

D'autres materias

Dna-binding proteins; Nuclear proteins; Molecular sequence data; Type 2; Genbank/af033342; Proliferator-activated receptor gamma; Messenger; Hyperinsulinism; Ratios; Monosaccharide transport proteins; Glucose transporter type 4; Macaca mulatta; Proliferator-activated receptor gamma 2; Ccaat-enhancer-binding proteins; Amino acid sequence; Muscle proteins; Cytoplasmic and nuclear; Hyperinsulinemia; Base sequence; Reverse transcriptase polymerase chain reaction; Genbank/af033343; Nucleotide sequences; Sequence homology; Active transport; Complementary; Molecular sequence data

Langue

anglais

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-27

Format: MODS

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Relationships of PPAR gamma and PPAR gamma 2 mRNA levels to obesity, diabetes and hyperinsulinaemia in rhesus monkeys

1998

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Informations bibliographiques