Detection and Quantification of Capsular Exopolysaccharides from Streptococcus thermophilus Using Lectin Probes
2006
Robitaille, G. | Moineau, S. | St-Gelais, D. | Vadeboncoeur, C. | Britten, M.
The aim of this work was to use fluorescently labeled lectins to develop a convenient and reliable method to determine the relative abundance of capsular polysaccharides (CPS) at the surface of Streptococcus thermophilus MR-1C cells. Fluorescein isothiocyanate-labeled peanut agglutinin isolated from Arachis hypogaea was found to interact specifically with the CPS of STREP: thermophilus MR-1C. This labeled lectin was then used as an effective probe to detect and quantify CPS. A fluorescence-based lectin-binding assay was successfully applied to follow the accumulation of CPS during the growth of STREP: thermophilus MR-1C in milk and in M17 broth supplemented with lactose. Our results showed that in both media, CPS production by STREP: thermophilus MR-1C began during the exponential phase of growth and continued for several hours after the culture reached the stationary growth phase.
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