Protein stapling via azide–alkyne ligation
2014
Abdeljabbar, Diya M. | Piscotta, Frank J. | Zhang, Siyan | James Link, A.
Here we demonstrate a methodology, termed protein stapling, for the introduction of covalent constraints into recombinant proteins. Using the azide–alkyne click reaction as the stapling chemistry, we have improved the thermostability of a model leucine zipper protein. Additionally, stapling the core of the small, globular protein G resulted in improved binding to its target, immunoglobulin G.
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