Protein stapling via azide–alkyne ligation

Abdeljabbar, Diya M.; Piscotta, Frank J.; Zhang, Siyan; James Link, A.

Protein stapling via azide–alkyne ligation

2014

Abdeljabbar, Diya M. | Piscotta, Frank J. | Zhang, Siyan | James Link, A.

Here we demonstrate a methodology, termed protein stapling, for the introduction of covalent constraints into recombinant proteins. Using the azide–alkyne click reaction as the stapling chemistry, we have improved the thermostability of a model leucine zipper protein. Additionally, stapling the core of the small, globular protein G resulted in improved binding to its target, immunoglobulin G.

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