Effect of ADP-ribosylation reaction on bull testis chromatin

Faraone-Mennella, M.R.; De Lucia, F.; Palomba, L.; Quesada, P.; Farina, B.

Effect of ADP-ribosylation reaction on bull testis chromatin

1993

Faraone-Mennella, M.R. | De Lucia, F. | Palomba, L. | Quesada, P. | Farina, B.

Polynucleosomes prepared from bull testis nuclei were characterized: DNA length, by agarose gel electrophoresis, and distribution of poly(ADP-ribose)polymerase activity, by incubation with 0.64mM NAD were determined. Maximal activity was found in nucleosome fractions of 3-5 units. Chromatin fragments (5-2 kbp polynucleosomes) were analysed by circular dichroism in both native and ADP-ribosylated forms. The spectrum of the endogenously ADP-ribosylated polynucleosomes, compared to the native fraction, exhibited a higher ellipticity value and a shift of the cross-over point towards the lowest wavelengths, behaving like an H1-depleted chromatin.

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Mots clés AGROVOC

adenosine diphosphate animals bulls cattle cell nucleus centrifugation chemistry chromatin chromatin dna metabolism spectral analysis testes testis ultrastructure

Informations bibliographiques

Publié dans

Biochemistry and molecular biology international

Volume 31 Numéro 2 Pagination 373 - 380 ISSN 1039-9712

D'autres materias

Circular dichroism; Post-transcription modification; Poly(adp-ribose) polymerases; Density gradient; Nucleosomes; Male; Adenosine diphosphate ribose; Protein conformation

Langue

anglais

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-28

Format: MODS

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