Effect of ADP-ribosylation reaction on bull testis chromatin

Faraone-Mennella, M.R.; De Lucia, F.; Palomba, L.; Quesada, P.; Farina, B.

Effect of ADP-ribosylation reaction on bull testis chromatin

1993

Faraone-Mennella, M.R. | De Lucia, F. | Palomba, L. | Quesada, P. | Farina, B.

Polynucleosomes prepared from bull testis nuclei were characterized: DNA length, by agarose gel electrophoresis, and distribution of poly(ADP-ribose)polymerase activity, by incubation with 0.64mM NAD were determined. Maximal activity was found in nucleosome fractions of 3-5 units. Chromatin fragments (5-2 kbp polynucleosomes) were analysed by circular dichroism in both native and ADP-ribosylated forms. The spectrum of the endogenously ADP-ribosylated polynucleosomes, compared to the native fraction, exhibited a higher ellipticity value and a shift of the cross-over point towards the lowest wavelengths, behaving like an H1-depleted chromatin.

Показать больше [+]

Ключевые слова АГРОВОК

adenosine diphosphate animals bulls cattle cell nucleus centrifugation chemistry chromatin chromatin dna metabolism spectral analysis testes testis ultrastructure

Библиографическая информация

Опубликовано в

Biochemistry and molecular biology international

Том 31 Выпуск 2 Нумерация страниц 373 - 380 ISSN 1039-9712

Другие темы

Circular dichroism; Post-transcription modification; Poly(adp-ribose) polymerases; Density gradient; Nucleosomes; Male; Adenosine diphosphate ribose; Protein conformation

Язык

Английский

Тип

Journal Article; Text

В АГРИСе с: 2024-02-28

Формат: MODS

Поставщик данных

Эту запись предоставил National Agricultural Library

Откройте коллекцию этого поставщика данных в AGRIS

Посмотрите в Google Scholar

If you notice any incorrect information relating to this record, please contact us at agris@fao.org agris@fao.org