Differentiation of avian adenovirus type-II strains by restriction endonuclease fingerprinting

Zhang, C.; Nagaraja, K.V.

Differentiation of avian adenovirus type-II strains by restriction endonuclease fingerprinting

1989

Zhang, C. | Nagaraja, K.V.

Three serologically indistinguishable viruses from the avian adenovirus type-II splenomegaly virus of chickens, marble spleen disease virus of pheasants, and hemorrhagic enteritis virus of turkeys, were analyzed by restriction endonuclease fingerprinting. The DNA from these viruses were examined with 6 restriction endonucleases (Bgl II, EcoRI, HindIII, Hha I, Xho I, and BamHI). Markedly different DNA cleavage patterns were found in these virus isolates with all the 5 enzymes, except with BamHI, suggesting genetic differences between isolates of adenovirus type II. Restriction endonuclease analyses were found to provide a method for distinguishing genetically different, and yet serologically similar, strains of avian adenovirus type II.

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Mots clés AGROVOC

adenoviridae analysis animals aviadenovirus classification differential diagnosis dna genetic variation genetics microbiology microscopy strain differences turkeys

Informations bibliographiques

Publié dans

American journal of veterinary research

Volume 50 Numéro 9 Pagination 1466 - 1470 ISSN 0002-9645

D'autres materias

Poultry diseases; Splenomegaly; Isolation & purification; Genetic techniques and protocols; Electron; Species specificity; Adenoviridae infections; Dna restriction enzymes; Viral; Nucleotide mapping; Marble spleen disease virus; Veterinary; Hemorrhagic enteritis

Langue

anglais

Type

Text; Journal Article

Sur AGRIS depuis: 2024-02-29

Format: MODS

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