Differentiation of avian adenovirus type-II strains by restriction endonuclease fingerprinting

Zhang, C.; Nagaraja, K.V.

Differentiation of avian adenovirus type-II strains by restriction endonuclease fingerprinting

1989

Zhang, C. | Nagaraja, K.V.

Three serologically indistinguishable viruses from the avian adenovirus type-II splenomegaly virus of chickens, marble spleen disease virus of pheasants, and hemorrhagic enteritis virus of turkeys, were analyzed by restriction endonuclease fingerprinting. The DNA from these viruses were examined with 6 restriction endonucleases (Bgl II, EcoRI, HindIII, Hha I, Xho I, and BamHI). Markedly different DNA cleavage patterns were found in these virus isolates with all the 5 enzymes, except with BamHI, suggesting genetic differences between isolates of adenovirus type II. Restriction endonuclease analyses were found to provide a method for distinguishing genetically different, and yet serologically similar, strains of avian adenovirus type II.

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Ключевые слова АГРОВОК

adenoviridae analysis animals aviadenovirus classification differential diagnosis dna genetic variation genetics microbiology microscopy strain differences turkeys

Библиографическая информация

Опубликовано в

American journal of veterinary research

Том 50 Выпуск 9 Нумерация страниц 1466 - 1470 ISSN 0002-9645

Другие темы

Poultry diseases; Splenomegaly; Isolation & purification; Genetic techniques and protocols; Electron; Species specificity; Adenoviridae infections; Dna restriction enzymes; Viral; Nucleotide mapping; Marble spleen disease virus; Veterinary; Hemorrhagic enteritis

Язык

Английский

Тип

Text; Journal Article

В АГРИСе с: 2024-02-29

Формат: MODS

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