COMPARISON OF SACCHARIFICATION METHODS FOR BIOTECHNOLOGICAL UTILIZATION OF WHEAT BRAN
2023
Pavel Divis | Jaromir Porizka | Jakub Nabelek | Vendula Hrabalova | Zuzana Slavikova
This study compares various methods used for the hydrolysis of lignocellulosic waste materials in terms of sugar yield and the content of inhibiting substances. Wheat bran was used as the lignocellulosic material, and the following hydrolysis methods were investigated: one-step alkaline hydrolysis, repeated alkaline hydrolysis, acid hydrolysis, and a combination of these methods with enzymatic hydrolysis. The combination of acid and enzymatic hydrolysis yielded the highest sugar yield, with a concentration of sugars in the hydrolyzate reaching up to 60 g/L and a yield of around 90%. The total concentration of phenolic substances, which can act as inhibitors during further biotechnological processing of the hydrolyzate, was approximately 270 mg/L GAE when using acid hydrolysis combined with enzymatic hydrolysis.
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