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Incidence of micronuclei in lymphocytes of pig in the high background radiation area (Cheongwon-gun and Boeun-gun)
2005
Lee, H.J. (Chonnam National University, Gwangju, Republic of Korea) | Kang, C.M. (Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea) | Kim, I.H. (Chungbuk National University, Cheongju, Republic of Korea) | Kim, T.H. (Kyungpook National University, Daegu, Republic of Korea) | Ryu, S.Y. (Chungnam National University, Daejeon, Republic of Korea) | Jo, S.K. (Korea Atomic Energy Research Institute, Daejeon, Republic of Korea) | Kim, S.R. (Chonnam National University, Gwangju, Republic of Korea) | Lee, J.H. (Chonnam National University, Gwangju, Republic of Korea) | Kim, J.S. (Chonnam National University, Gwangju, Republic of Korea) | Kim, J.C. (Chonnam National University, Gwangju, Republic of Korea) | Kim, S.H. (Chonnam National University, Gwangju, Republic of Korea), E-mail: shokim@chonnam.ac.kr | Choi, S.Y. (Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea)
Cytogenetic and hematological analysis was performed in peripheral blood obtained from pigs bred in the high background radiation areas (HBRA) (Cheongwon-gun and Boeun-gun) and a control area. The frequencies of gamma-ray induced micronuclei (MN) in the cytokinesis-blocked (CB) lymphocytes at several doses were measured in three pigs. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the swine lymphocytes exposed in vitro to radiation over the range from 0 mGy to 1,969 mGy.
Afficher plus [+] Moins [-]Evaluation of micronucleus frequency in cytokinesis-blocked lymphocytes of cattle in the vicinity of Uljin nuclear power station
Kim, S.R.;Kim, S.H.(Chonnam National University, Gwangju, Republic of Korea)E-mail:shokim@chonnam.ac.kr | Kang, C.M.(Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea)
Cytogenetic and hematological analysis was performed in peripheral blood of cattle in the vicinity of Uljin nuclear power station and control area. The frequency of micronuclei(MN) in peripheral blood lymphocytes from cattle was used as a biomarker of radiobiological effects resulting from exposure to environmental radiation. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the bovine lymphocytes exposed in vitro to radiation over the range from 0 Gy to 4 Gy.
Afficher plus [+] Moins [-]Peripheral lymphocyte counts in Holstein-Friesian cattle infected with bovine leukemia virus in Korea
2005
Suh, G.H. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea), E-mail: ghsuh@rda.go.kr | Lee, C.G. (Chonnam National University, Gwangju, Republic of Korea) | Lee, J.C. (Seojeong College, Yangju, Republic of Korea) | Hur, T.Y. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Kang, S.J. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Son, D.S. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Ahn, B.S. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Kim, N.C. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Lee, C.Y. (Chonnam National University, Gwangju, Republic of Korea)
Hematologic investigations were made on the blood samples taken from bovine leukemia virus (BLV)-seropositive Holstein-Friesian cattle in Korea, and their absolute lymphocyte count was compared with that of BLV-seronegative cattle. The incidence of persistent lymphocytosis (PL) was also determined. The normal bovine lymphocyte count was established on the basis of studies of 656 blood samples taken three times from 297 seronegative animals aged from 0~6 months to over 5 years at 5~6-month intervals. The data were examined according to 7 age groups of samples placed into their respective age groups.
Afficher plus [+] Moins [-]Analysis of gamma-ray-induced DNA damage in human, mouse and rat peripheral blood lymphocytes using single-cell gel electrophoresis
Oh, H.;Jung, U.H.;Park, H.R.;Jo, S.K.(Korea Atomic Energy Research Institute, Daejeon, Republic of Korea)E-mail:skjo@kaeri.re.kr | Kim, S.H.(Chonnam National University, Gwangju, Republic of Korea)
The alkaline single-cell gel electrophoresis (SCGE) assay, called the comet assay, has been applied to detect DNA damage induced by a number of chemicals and biological factors in vivo and in vitro. The DNA damage was analysed by tail moment (TM) and tail length (TL), which were markers of DNA strand breaks in SCGE. Human, mouse and rat peripheral blood lymphocytes (PBLs) were irradiated with different doses of ∨60Co γ-rays, e.g. 1, 2, 4, and 8 Gy at a dose rate of 1 Gy/min. A dose-dependent increase in TM (p less than 0.01) and TL (p less than 0.01) was obtained at all the radiation doses (1-8 Gy) in human, mouse and rat PBLs.
Afficher plus [+] Moins [-]Establishment of micronuclus assay as biological dosimetry in pig lymphocytes after gamma-irradiation
Kim, S.R.;Lee, H.J.;Lee, J.H.;Kim, J.C.;Kim, S.H.(Chonnam National University, Gwangju, Republic of Korea)E-mail:shokim@chonnam.ac.kr | Kang, C.M.(Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea) | Kim, T.H.(Kyungpook National University, Daegu, Republic of Korea) | Jo, S.K.(Korea Atomic Energy Research Institute, Daejeon, Republic of Korea)
The purpose of the present experiment was to investigate the micronuclei(MN) frequency in cytokinesis-blocked(CB) cells after various doses of gamma-rays in pig (Landrace, male, 3-month-old) and so to contribute to the clarification of the question whether these species are suitable as a target organism in the test system. The frequencies of binucleated cells, and gamma-ray-induced MN in CB cells at several doses were measured in three donors. The peaks of binucleated lymphocyte formation(22%) were found at a concentration of 2% phytohaemagglutinin(PHA) and 4 ㎍/ml Cytochalasin B(Cyt-B) in pig at 72 hours after incubation.
Afficher plus [+] Moins [-]Immunotoxic effects of Vomitoxin
1992
Kim, J.S. | Kim, Y.H. (Gyeongsang Nat'l Univ., Chinju (Korea Republic). Coll. of Veterinary Medicine)
Morphological studies on the hemal node in the sika deer
1991
Yoon, Y.S. | Han, J.H. (Kangweon National Univ.,Chuncheon (Korea Republic). Dept. of Veterinary Medicine)
Studies on enzootic bovine leukosis - (1) - The changes of antibodies against bovine leukemia virus and peripheral blood lymphocyte on Holstein cattle in a dairy farm
1990
Kim, C.J. | Son, J.Y. | Ko, K.W. (Yeungnam University, Kyeongsan (Korea Republic). Department of Animal Science)
Effect of Euonymus alatus and Ulmus clavidiana var japonica on the immune system
1994
Kim, J.M. | Cho, J.G. | Jung, Y.M. | Park, T.W. (Chonbuk National University, Chonju (Korea Republic). College of Veterinary Medicine) | Choi, M.S. (Kunsan National University, Kunsan (Korea Republic). College of Ocean Science and Technology)
Effect of Bupleurum falcatum on the immune system
1994
Cho, J.G. | Kim, J.M. (Chonbuk National University, Chonju (Korea Republic). College of Veterinary Medicine)
