The approval of use of certain food-grade phosphates as food additives in a wide variety of meat products greatly stimulated research on the applications of phosphates in foods. Although phosphates have never been classified as antimicrobial agents, a number of investigators have reported that phosphates have antimicrobial activities. Phytic acid is a natural plant inositol hexaphosphate constituting 1-5% of most cereals, nuts, legumes, oil seeds, pollen, and spores. In this study, we investigated antibacterial activities of sodium phytate (SPT), sodium pyrophosphate (SPP), sodium tripolyphosphate (STPP) on Salmonella typhimurium in tryptic soy broth and in row meat media including chicken, pork and beef.

A 4-year-old spayed female, Yorkshire terrier dog with a history of petechial and ecchymotic hemorrhages on the face, trunk and hind limb was referred to Veterinary Medical Teaching Hospital, Cheju National University. The complete blood count revealed a marked thrombocytopenia (96×10³/㎕). The biochemical profile showed only slightly increased glucose. The coagulation profile such as prothrombin time and activated partial thromboplastin time, was within the reference range. In Giemsa staining, there were no endoparasites like as Babesia spp. on the RBC.

A 2-year-old spayed female ferret with the clinical signs of diarrhea and anorexia for about 8 days was presented to Hansung animal hospital. The diarrhea was black and paste form. Three palpable abdominal masses were detected in physical examination. By cytologic examination using fine need aspiration, the patient was tentatively diagnosed as lymphoma. Chemotherapy was started with prednisone, vincristine and cyclophosphamide. However, the client requested stopping the therapy at day 18 and the ferret was euthanized.

To determine the effects of anesthesia to clove oil on the secondary stress indices, changes in haematological and blood chemistry were monitored in healthy crucian carps (Carassius auratus). 24 fishes were divided into three groups, and blood was collected before anaesthesia (control group), immediately after anaesthesia at a concentration of 30 mg/l clove oil (anesthetized group), and 24 h after anaesthesia (recovered group). The anesthesia to clove oil significantly increased glucose in anaesthetized group, and constantly decreased lactate dehydrogenase in anaesthetized group and recovered group.

The cause and pathogenesis of inflammatory bowel disease remain unknown and no definite therapy exists until now. The present study was conducted to investigate the anti-inflammatory effects of a herbal preparation (HemoHIM) in colitis induced by 30 mg of trinitrobenzene sulfonic acid (TNBS) in rats. Sprague-Dawley rats were divided into 5 groups. Each group was treated with 1 mg of HemoHIM/ml of drinking water, 4 mg of HemoHIM/ml of drinking water, 50 mg of HemoHIM/kg of body weight (i.p. once every other day) or 100 mg/kg of HemoHIM of body weight (i.p. once every other day) from the next day.

Streptococcus spp. comprising Streptococcus (S.) uberis and S. dysgalactiae strains is major cause of bovine mastitis from particularly well-managed or low somatic cell count herds that have successfully controlled contagious pathogens. In this study, antimicrobial susceptibility and genetic characteristics of S. uberis isolated from clinical or subclinical mastitis milk at 2003 were investigated. Eighty seven isolates of Streptococcus spp. were identified by the conventional biochemical methods. The antimicrobial susceptibility by disk diffusion method was determined for 46 S. uberis, 11 S. bovis, 10 S. oralis, 6 S. uberis and 14 other Streptococcus spp., Overall, the tested strains were susceptible to tetracycline (11.5%), amikacin (14.9%), streptomycin (16.1%), neomycin (26.4%), kanamycin (35.6%), gentamicin (65.2%), oxacillin (70.1%), ampicillin (75.9%), chloramphenicol (78.2%), and cephalothin (97.7%).

To establish a statistically reliable sampling strategy for serological surveillance of classical swine fever (CSF) in Korea, antibody test data from CSF surveillance conducted during year 2005 were analyzed. The most appropriate sampling method was determined to be stratified multi-stage random sampling strategy, in which the primary sampling unit is a pig farm and the secondary are the pigs by the strata of breeders and finishers in the selected farm. The optimum sample size was 5 to 19 including 1 to 2 breeders according to the number of pigs in the farm.

A case of multicentric high grade T cell lymphoma is reported in a 5-year-old male Maltese dog with generalized lymphadenopathy. The dog showed depression, anorexia, blindness, jaundice, arrhythmia, and hematuria for 8 months. Complete blood count and chemistry profile revealed anemia and increased alanine transferase, alkaline phosphatase, total bilirubin, and total cholesterol. Grossly, most of lymph nodes, spleen, and liver were enlarged and neoplastic masses were occupied in these tissues. Histologically, massive accumulation of small noncleaved neoplastic lymphocytes with high mitotic figures was observed in all lymph nodes and spleen.

A 3 year-old female Afghan hound came to the Veterinary Referral Hospital of the College of Veterinary Medicine, Seoul National University for artificial insemination (AI) with frozen semen. In order to inseminate, semen was frozen in USA 3 years ago. Frozen semen was sent by air from Santiago to Seoul for AI. The stud died 2 years ago, so we could only use a limited amount of frozen semen in that estrus cycle. The number of total motile spermatozoa was 59.4×10∨6 and the total volume was 1.2 ml. The frozen spermatozoa were thawed in 70℃ water for 8 sec, which were then deposited at the bilateral uterine horns by a surgical method.

Lactic acid bacteria (LAB) has been regarded as a useful microorganism and tried to manipulate plasmid DNA for increasing the usefulness. Although several methods have been developed is isolate plasmid DNA from Escherichia coli (E. coli), these methods were not sufficient to apply to LAB with exception of O'Sullivan's lysis method. So, we evaluated plasmid DNA extraction from LAB using general E. coli preparation methods and tried to improve the extraction yield and DNA purity by modifying O'Sullivan's alkaline lysis method.