Affiner votre recherche
Résultats 231-240 de 991
Cloning and expression of NS3 gene of Pakistani isolate type 2 dengue virus Texte intégral
2018
Yasmin, Farkhanda | Yaqub, Tahir | Idrees, Muhammad | Shahzad, Wasim | Hashmi, Abu Saeed | Aqil, Kiran | Mukhtar, Nadia | Zahoor, Muhammad Yasir | Akhtar, Naeem | Umar, Sajid
Cloning and expression of NS3 gene of Pakistani isolate type 2 dengue virus Texte intégral
2018
Yasmin, Farkhanda | Yaqub, Tahir | Idrees, Muhammad | Shahzad, Wasim | Hashmi, Abu Saeed | Aqil, Kiran | Mukhtar, Nadia | Zahoor, Muhammad Yasir | Akhtar, Naeem | Umar, Sajid
Dengue is one of the major emerging viral diseases in the world, with dramatic increases in reported cases in the last few decades and annual worldwide occurrence of approximately 390 million infections. It is a highly important mosquito-vectored disease and is a problem in tropical and subtropical areas of the world. The major aim of this study was to clone and express the dengue NS3 gene, in service to its therapeutic importance for the development of stable cell lines. Blood samples from dengue fever (DF) patients were collected and subjected to PCR amplification of the NS3 gene of dengue virus serotype-2 (DENV-2). The NS3 gene was amplified using gene specific primers and cloned in the TA cloning vectors. The gene was successfully expressed in mammalian expression vector pcDNA3.1. The current finding was different from a previously reported DENV-2 strain replicon constructed in different cells, in which the whole genetic material of the virus was used instead of an active protease gene, and which gave a low yield of replicon expressing cells. Recombinant NS3 could be used to produce an antibody that is possibly helpful for developing a single step diagnostic assay to detect the dengue virus NS3 antigen in sera of dengue patients.
Afficher plus [+] Moins [-]Cloning and expression of NS3 gene of Pakistani isolate type 2 dengue virus Texte intégral
2018
Yasmin Farkhanda | Yaqub Tahir | Idrees Muhammad | Shahzad Wasim | Hashmi Abu Saeed | Aqil Kiran | Mukhtar Nadia | Zahoor Muhammad Yasir | Akhtar Naeem | Umar Sajid
Dengue is one of the major emerging viral diseases in the world, with dramatic increases in reported cases in the last few decades and annual worldwide occurrence of approximately 390 million infections. It is a highly important mosquito-vectored disease and is a problem in tropical and subtropical areas of the world. The major aim of this study was to clone and express the dengue NS3 gene, in service to its therapeutic importance for the development of stable cell lines.
Afficher plus [+] Moins [-]Histamine content in rennet ripening cheeses during storage at different temperatures and times Texte intégral
2018
Madejska, Anna | Michalski, Mirosław | Pawul-Gruba, Marzena | Osek, Jacek
Histamine content in rennet ripening cheeses during storage at different temperatures and times Texte intégral
2018
Madejska, Anna | Michalski, Mirosław | Pawul-Gruba, Marzena | Osek, Jacek
In recent years, there has been a great interest in biogenic amines such histamine, as they are associated with the quality and safety of some kinds of fermented foods. The aim of this study was to evaluate the effect of temperature and storage time on the content of histamine in cheeses. Samples of mould and hard cheeses were examined with RP-HPLC with an organic-aqueous mobile phase containing acidic buffer and chaotropic salt. The samples were stored either at 22 ± 2°C for 42 days (mould and hard cheeses) or at 4 ± 2°C for 112 days (mould cheeses) and 133 days (hard cheeses). The mean total histamine content in cheeses stored at 22°C was higher than the content in those stored at 4°C, with the highest concentrations found in Gorgonzola Piccante cheese (730.47 mg/kg). Histamine concentration in some types of cheeses exceeded the toxic threshold dose, indicating that after long or inadequately cool storage they may not be safe for consumers. To protect cheeses from contamination with histamine-producing bacteria and to safeguard consumers from poisoning, factors conducive to this amine’s formation should be minimised during cheese processing. Suitable temperature and time during storage of cheeses are recommended to avoid the intoxication. Monitoring of this toxin in food is necessary to ensure safety of consumers.
Afficher plus [+] Moins [-]Histamine content in rennet ripening cheeses during storage at different temperatures and times Texte intégral
2018
Madejska Anna | Michalski Mirosław | Pawul-Gruba Marzena | Osek Jacek
In recent years, there has been a great interest in biogenic amines such histamine, as they are associated with the quality and safety of some kinds of fermented foods. The aim of this study was to evaluate the effect of temperature and storage time on the content of histamine in cheeses.
Afficher plus [+] Moins [-]Effects of newly developed synbiotic and commercial probiotic products on the haematological indices, serum cytokines, acute phase proteins concentration, and serum immunoglobulins amount in sows and growing pigs – a pilot study Texte intégral
2018
Czyżewska-Dors, Ewelina | Kwit, Krzysztof | Stasiak, Ewelina | Rachubik, Jarosław | Śliżewska, Katarzyna | Pomorska-Mól, Małgorzata
Effects of newly developed synbiotic and commercial probiotic products on the haematological indices, serum cytokines, acute phase proteins concentration, and serum immunoglobulins amount in sows and growing pigs – a pilot study Texte intégral
2018
Czyżewska-Dors, Ewelina | Kwit, Krzysztof | Stasiak, Ewelina | Rachubik, Jarosław | Śliżewska, Katarzyna | Pomorska-Mól, Małgorzata
The aim of the study was to determine the effects of supplementation of sows’ and growing pigs’ diets with three newly developed synbiotic and two extant commercial probiotic products on selected immune parameters under field conditions. The study was performed on 30 sows and 48 piglets of the Danbred breed. Immune parameters such as concentration and proportion of white blood cells and their subpopulations, immunoglobulins amount in serum, and serum concentration of cytokines and acute phase proteins were recorded with the use of a haematology analyser and ELISA kits. No significant differences between treatment groups and controls were found with regard to the immune parameters evaluated except for serum immunoglobulin concentration, which was significantly increased by synbiotic products B and C and probiotic product D. The results of the study indicate that the synbiotic products B and C and probiotic product D are worthy of further investigation as promising candidates to improve the immune status of healthy sows and their offspring.
Afficher plus [+] Moins [-]Effects of newly developed synbiotic and commercial probiotic products on the haematological indices, serum cytokines, acute phase proteins concentration, and serum immunoglobulins amount in sows and growing pigs – a pilot study Texte intégral
2018
Czyżewska-Dors Ewelina | Kwit Krzysztof | Stasiak Ewelina | Rachubik Jarosław | Śliżewska Katarzyna | Pomorska-Mól Małgorzata
The aim of the study was to determine the effects of supplementation of sows’ and growing pigs’ diets with three newly developed synbiotic and two extant commercial probiotic products on selected immune parameters under field conditions.
Afficher plus [+] Moins [-]Toxicological evaluation of flumequine in pubertal male rats after oral administration for six weeks Texte intégral
2018
Kang, JeongWoo | Hossain, Md Akil | Choi, Byungkook | Cho, Joon-Hyoung | Kang, Seok-Jin | Ku, Hyun-Ok | Jeong, Sang-Hee | Kang, Hwan-Goo
Toxicological evaluation of flumequine in pubertal male rats after oral administration for six weeks Texte intégral
2018
Kang, JeongWoo | Hossain, Md Akil | Choi, Byungkook | Cho, Joon-Hyoung | Kang, Seok-Jin | Ku, Hyun-Ok | Jeong, Sang-Hee | Kang, Hwan-Goo
Veterinarians use flumequine (FLU) widely but its toxicological effects are still unclear. FLU doses of 53, 200, or 750 mg/kg were administered orally for six weeks to pubertal male rats for evaluation of their toxicity. Weight gain was poorer after seven days of exposure to FLU 750, but relative weights of the brain, adrenal and thyroid glands, and testes were notably higher. Haematological and lipid profile parameters, cardiac markers, and inorganic phosphate significantly increased in the FLU 750 group. Blood glucose, oestradiol and serum concentrations of immunoglobulins G (IgG) and E (IgE) significantly decreased after treatment. The levels of interleukins 10 (IL-10) and 6 (IL-6) fell significantly in the FLU 200 and FLU 750 groups. Cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) and cyclooxygenase-2 (Cox-2) expression amplified after treatment. Serum levels of free triiodothyronine (fT3) and free thyroxine (fT4) reduced in the FLU 200 and FLU 750 groups without changes in total T3 or T4 level. All doses of FLU significantly depressed concentrations of thyroid-stimulating hormone (TSH) and testosterone. Histopathology of thyroid glands from rats treated with FLU 750 showed degeneration and depletion of thyroid follicular epithelial cells. Expression of 8-hydroxydeoxyguanosine (8-OHdG) was increased in a dose-dependent manner in the brain, but decreased in the testes. Expression of CYP1A1 increased in the adrenal and pituitary glands. The results of this study suggest that the toxicity of FLU in rats is an effect of its disruptive influence on the pituitary-thyroid hormonal system and on the dysfunction of the immune system.
Afficher plus [+] Moins [-]Toxicological evaluation of flumequine in pubertal male rats after oral administration for six weeks Texte intégral
2018
Kang JeongWoo | Hossain Md Akil | Choi Byungkook | Cho Joon-Hyoung | Kang Seok-Jin | Ku Hyun-Ok | Jeong Sang-Hee | Kang Hwan-Goo
Veterinarians use flumequine (FLU) widely but its toxicological effects are still unclear.
Afficher plus [+] Moins [-]Analysis of haematological and biochemical blood parameters after electrical cardioversion of atrial fibrillation in dogs Texte intégral
2018
Noszczyk-Nowak, Agnieszka | Michałek, Marcin | Janiszewski, Adrian | Kurosad, Agnieszka | Sławuta, Agnieszka | Cepiel, Alicja | Pasławska, Urszula
Analysis of haematological and biochemical blood parameters after electrical cardioversion of atrial fibrillation in dogs Texte intégral
2018
Noszczyk-Nowak, Agnieszka | Michałek, Marcin | Janiszewski, Adrian | Kurosad, Agnieszka | Sławuta, Agnieszka | Cepiel, Alicja | Pasławska, Urszula
Electrical cardioversion is a therapeutic procedure used to convert various types of arrhythmias back to sinus rhythm. It is used to restore the sinus rhythm in dogs with atrial fibrillation. The effect of the electrical energy used during cardioversion on red blood cells (RBC) is not fully understood. Studies on humans reported lysis of RBC following electrical cardioversion. Similar studies have not been carried out on dogs. The aim of the study was to assess the effect of electrical cardioversion on chosen RBC parameters. The study was carried out on 14 large and giant breed dogs weighing from 30 to 84 kg with lone atrial fibrillation (lone AF). Electrical cardioversion was carried out under general anaesthesia by biphasic shock with 70–360 J of energy. Blood was collected at T0 – during atrial fibrillation, prior to cardioversion, and at T1 – 30 min after electrical cardioversion. Complete blood counts as well as total and direct bilirubin concentrations were evaluated. A maximum output of 360 J was used. In all cases, electrical cardioversion was effective, and no significant changes in the number of RBC and RBC indices were noted. Similarly, there were no statistically significant differences in the levels of total and direct bilirubin. Electrical cardioversion in dogs led neither to statistically nor clinically significant RBC lysis.
Afficher plus [+] Moins [-]Analysis of haematological and biochemical blood parameters after electrical cardioversion of atrial fibrillation in dogs Texte intégral
2018
Noszczyk-Nowak Agnieszka | Michałek Marcin | Janiszewski Adrian | Kurosad Agnieszka | Sławuta Agnieszka | Cepiel Alicja | Pasławska Urszula
Electrical cardioversion is a therapeutic procedure used to convert various types of arrhythmias back to sinus rhythm. It is used to restore the sinus rhythm in dogs with atrial fibrillation. The effect of the electrical energy used during cardioversion on red blood cells (RBC) is not fully understood. Studies on humans reported lysis of RBC following electrical cardioversion. Similar studies have not been carried out on dogs. The aim of the study was to assess the effect of electrical cardioversion on chosen RBC parameters.
Afficher plus [+] Moins [-]Pyrrolizidine alkaloids in honey: determination with liquid chromatography-mass spectrometry method Texte intégral
2018
Kowalczyk, Ewelina | Kwiatek, Krzysztof
Pyrrolizidine alkaloids in honey: determination with liquid chromatography-mass spectrometry method Texte intégral
2018
Kowalczyk, Ewelina | Kwiatek, Krzysztof
Pyrrolizidine alkaloids (PAs) are probably the most widespread toxins of natural origin. More than 6,000 plant species produce these toxic compounds. Bees can forage on flowers of plants producing PAs, which leads to contamination of honey with the toxic compounds. To determine the contamination of honey with PAs, a sensitive method based on liquid chromatography coupled with mass spectrometry has been developed. PAs were extracted with 0.05 M sulphuric acid and purified with MCX cartridges. A solvent mixture consisting of ethyl acetate, methanol, acetonitrile, ammonia, and triethylamine (8:1:1:0.1:0.1, v/v) was used to wash alkaloids from the cartridges. After evaporation the residues were reconstituted in water and methanol mixture and subjected to LC–MS analysis. The developed method was validated according to SANTE/11945/2015 requirements. The recovery was from 80.6% to 114.5%. The repeatability ranged from 2.3% to 14.6%, and the reproducibility was from 4.9% to 17.7%. A new method for the determination of PAs in honey has been developed and validated. All evaluated parameters were in accordance with the SANTE/11945/2015 guidance document. Out of 50 analysed honey samples, 16 (32%) were positive for the content of at least one PA.
Afficher plus [+] Moins [-]Pyrrolizidine alkaloids in honey: determination with liquid chromatography-mass spectrometry method Texte intégral
2018
Kowalczyk Ewelina | Kwiatek Krzysztof
Pyrrolizidine alkaloids (PAs) are probably the most widespread toxins of natural origin. More than 6,000 plant species produce these toxic compounds. Bees can forage on flowers of plants producing PAs, which leads to contamination of honey with the toxic compounds. To determine the contamination of honey with PAs, a sensitive method based on liquid chromatography coupled with mass spectrometry has been developed.
Afficher plus [+] Moins [-]GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum Texte intégral
2018
Taşçı, Serap Koral | Bingöl, Seyit Ali
GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum Texte intégral
2018
Taşçı, Serap Koral | Bingöl, Seyit Ali
Glucagon-like peptide-1 (GLP-1) is a polypeptide that is mainly produced by intestinal L cells and is encoded by the proglucagon gene. In this study, GLP-1 localisation was investigated in the ileum of healthy and diabetic mice by immunohistochemistry and proglucagon gene expression was assayed by reverse transcription-polymerase chain reaction. This study included 18 male Balb/c mice that were divided into diabetic, sham, and control groups. Mice in the diabetic group received 100 mg/kg of streptozotocin. Immunohistochemical expression of GLP-1 was determined using the avidin–biotin–peroxidase complex technique, and proglucagon gene expression was determined by RT-PCR. Analysis of GLP-1 immunohistochemical localisation showed that GLP-1-immunopositive cells (L cells) were present between epithelial cells in the intestinal crypts. The intensity and localisation of GLP-1 immunoreactivity were similar among the mice in all the groups. Proglucagon gene expression levels were also statistically similar among the mice in all the groups. No difference was demonstrated among the mice in the diabetic, sham, or control groups with respect to proglucagon gene expression and GLP-1 localisation in the ileum, suggesting that diabetes does not affect proglucagon gene expression in the ileum.
Afficher plus [+] Moins [-]GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum Texte intégral
2018
Taşçı Serap Koral | Bingöl Seyit Ali
Glucagon-like peptide-1 (GLP-1) is a polypeptide that is mainly produced by intestinal L cells and is encoded by the proglucagon gene. In this study, GLP-1 localisation was investigated in the ileum of healthy and diabetic mice by immunohistochemistry and proglucagon gene expression was assayed by reverse transcription-polymerase chain reaction.
Afficher plus [+] Moins [-]Inactivated H5 antigens of H5N8 protect chickens from lethal infections by the highly pathogenic H5N8 and H5N6 avian influenza viruses Texte intégral
2018
Jin, Myongha | Jang, Yunyueng | Seo, Taehyun | Seo, Sang Heui
Inactivated H5 antigens of H5N8 protect chickens from lethal infections by the highly pathogenic H5N8 and H5N6 avian influenza viruses Texte intégral
2018
Jin, Myongha | Jang, Yunyueng | Seo, Taehyun | Seo, Sang Heui
Introduction: Highly pathogenic Asian H5-subtype avian influenza viruses have been found in poultry and wild birds worldwide since they were first detected in southern China in 1996. Extensive control efforts have not eradicated them. Vaccination prevents such viruses infecting poultry and reduces the number lost to compulsory slaughter. The study showed the efficacy of inactivated H5 vaccine from the H5N8 virus against highly pathogenic H5N8 and H5N6 avian influenza viruses in chickens. Material and Methods: Reverse genetics constructed an H5 vaccine virus using the HA gene of the 2014 H5N8 avian influenza virus and the rest of the genes from A/PR/8/34 (H1N1). The vaccine viruses were grown in fertilised eggs, partially purified through a sucrose gradient, and inactivated with formalin. Chickens were immunised i.m. with 1 µg of oil-adjuvanted inactivated H5 antigens. Results: Single dose H5 vaccine recipients were completely protected from lethal infections by homologous H5N8 avian influenza virus and shed no virus from the respiratory or intestinal tracts but were not protected from lethal infections by heterologous H5N6. When chickens were immunised with two doses and challenged with homologous H5N8 or heterologous H5N6, all survived and shed no virus. Conclusion: Our results indicate that two-dose immunisations of chickens with H5 antigens with oil adjuvant are needed to provide broad protection against different highly pathogenic H5 avian influenza viruses.
Afficher plus [+] Moins [-]Inactivated H5 antigens of H5N8 protect chickens from lethal infections by the highly pathogenic H5N8 and H5N6 avian influenza viruses Texte intégral
2018
Jin Myongha | Jang Yunyueng | Seo Taehyun | Seo Sang Heui
Introduction: Highly pathogenic Asian H5-subtype avian influenza viruses have been found in poultry and wild birds worldwide since they were first detected in southern China in 1996. Extensive control efforts have not eradicated them. Vaccination prevents such viruses infecting poultry and reduces the number lost to compulsory slaughter. The study showed the efficacy of inactivated H5 vaccine from the H5N8 virus against highly pathogenic H5N8 and H5N6 avian influenza viruses in chickens.
Afficher plus [+] Moins [-]Ameliorative effects of Helianthus annuus against nephrotoxic, cardiac, and haematological disorders in alloxan-induced hyperglycaemia in albino rats Texte intégral
2018
Onoja Samuel Okwudili | Udem Samuel Chukwuneke | Anaga Aruh Ottah
The study evaluated the ameliorative effects of Helianthus annuus leaf extract on nephrotoxicity, cardiac, and haematologic disorders in alloxan-induced hyperglycaemic rats.
Afficher plus [+] Moins [-]Complete genome sequences of three sub-genotype 2.1b isolates of classical swine fever virus in China Texte intégral
2018
Liu Chunxiao | Li Mingliang | Yin Xingwang | Zhang Hongliang | Xiang Lirun | Zhai Hongyue | Wang Congcong | Kan Yunchao | Yao Lunguang | Tian Zhijun | Leng Chaoliang
Classical swine fever (CSF) has caused severe economic losses in pig production in many countries. Recent CSF outbreaks in China are mainly associated with sub-genotype 2.1 of CSF virus (CSFV). Although there is abundant information regarding 2.1 isolates, few data are available on whole-genome analysis.
Afficher plus [+] Moins [-]