Serological study and isolation of Brucellae from dairy cattle were carried out in Kyungbook area of Korea during the period from 1984 to 1985. Biotyping of the isolates was done. Of the 11168 cattle in 2479 herds examined, 27 cattle (0.24%) in the 17 herds (o.69%) were serologically positive for brucellosis. Brucella abortus was recovered from 20 (74.1%) of the 27 infected cattle, and all the strains of the isolates belonged to biotype 1. Of the tissues cultured for brucellae, the supramammary lymph nodes had the highest rate (55.6%) of recovery of brucella organisms

Normal swine brains at 1 to 70 days after birth were used to investigate the presence and morphology of the subependymal layer (SL) in the ventricle walls. The brain samples were taken from 27 pigs of 4 swine breeds. SLs were observed on the walls of the lateral ventricle (LV) but none were observed on the walls of the 3th and 4th ventricles. SLs of the LV walls were composed of mainly 3-to 10-cell layers in thickness. The thinnest region of SLs was composed of only 1-to 2-cell thick on the dorsal and ventral walls, and the thickest region was composed of 250-to 300-cell thick on extension region of the SLs into the angle between the corpus callosum and caudate nucleus. SLs were extensively thick in young age but gradually decreased in size and cell number with age after 20-day age. SL layers were composed of mainly oligodendrocytes, astrocytes and immature cells of them. Morphological differences of SL in different breeds of pigs were not observed.

Observations were made on the blood picture of 50 multiparous and 98 infertile cows. Mean values of RBC, Hb, PVC, MCV, MCH, MCHC and platelets for normal multiparous cows were 6.87+-0.84X10** (6)/mm** (3), 10.3+-1.3g/100ml, 31.4+-3.7ml/100ml, 46.1+-6.9u** (3), 15.3+-2.6pg, 34+-2.7%, and 372.7+-304.7X10** (3)/mm** (3) respectively. Mean values of RBC, Hb, PVC, MCV, MCH, MCHC and platelets for infertile cows were 7.00+-0.98X10** (6)/mm** (3), 10.6+-1.2g/100ml, 32.1+-3.6ml/100ml, 45.8+-6.7u** (3), 15.3+-1.8pg, 33.9+-3.3%, and 382.7+-157.5X10** (3)/mm** (3), respectively. Mean values of WBC count for normal multiparous and infertile cows were 7.92+-1.72 and 9.04+-2.87X10** (3)/mm** (3) respectively. In the differential leukocytes count, mean values of neutrophils, lymphocytes, monocytes, eosinophils and basophils for normal multiparous cows were 29.7+-7.9%, 56.8+-7.6%, 2.9+-1.9%, 9.9+-5.3% and 0.3+-0.7%, respectively, and mean values of neutrophils, lymphocytes, monocytes, eosinophils and basophils for infertile cows were 31.9+-7.8%, 57.5+-7.9%, 2.6+-1.9%, 7.5+-4.8%, and 0.4+-0.2%, respectively

In order to investigate the morphological characteristics of dendritic cells in the mammary gland of the mouse (C57 BL/6), rat (W), rabbit and cat, the fine structures of the dendritic cells have been observed by the electron microscope. The dendritic cells with the well-developed processes had an irregular shape, and lacked the desmosome. The pinocytotic vesicles and tubular invaginations of the cell membrane were frequently observed, and the mitochondria with the well-developed cristae were located in the restricted region in the cytoplasm of the dendritic cells. The nuclei of dendritic cells were indented. In the mice and rats, the dendritic cells had a few langerhans cell granules (Birbeck granules). From the above results, it is confirmed that the ATPase-positive dendritic cells in the mammary gland are the langerhans cells

The present study was conducted in order to get the normal hematologic values of Korean Jindo dogs. Blood samples were taken from 328 (male 92, female 236) healthy Jindo dogs in Jindo area. The mean values of red blood cell (RBC) count, hemoglobin (Hb) content and packed cell volume (PCV) in the age group of less than one year were 6.77(male 7.44, female 6.76)X10** (6)/ul, 13,14(male 13.53, female 12.99)g/100ml and 43.28(male 44.47, female 42.79)ml/100ml, respectively, whereas the RBC count, Hb content and PCV in the age group of one year and more were 7.42(male 7.44, female 7.42)X10** (6)/ul, 14.98(male 15.56, female 14.76)g/100ml and 47.18(male 48.43, female 46.71)ml/100ml, respectively. The values of RBC count, Hb content and PVC appeared to be higher in the male than in the female and it increased with aging in the age group of less than one year. The mean values of mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin content (MCHC) in the age group of less than one year were 63.93(male 65.40, female 63.30)fl, 19.41(male 19.90, female 19.22) pg and 30.36(male 30.43, female 30.36)g/100ml respectively, whereas the MCV, MCH and MCHC in the age group of one year and more were 63.58(male 65.09, female 62.95)fl, 20.19(male 20.91, female 19.89) pg and 31.75(male 32.13, female31.60)g/100ml respectively. The values of MCV, MCH and MCHC appeared to be higher in the male than in the female. No differences were found in the MCV and MCH between age groups. The MCHC appeared to be higher in older age group. Mean platelet count in the age group of less than one year was 377,391(male 398,778, female 368,721)/ul, whereas mean platelet count in the age group of one year and more was 354,657(male 373,660, female 347,512)/ul. The platelet count appeared to be higher in the male than in the female and especially lower in the age group of three years and more (305,513/ul) than in the other age groups

This experiment was carried out to determine the progesterone concentration of bovine plasma by liquid phase double antibody enzyme immunoassay. The optimum conditions of assay-system, double (first and second) antibody and carrier (normal rabbit serum) were investigated. The optimum dilution rate of first antibody, second antibody and normal rabbit serum was 10X10** (3) to 15X10** (3), 20 and 1X10** (3) times, respectively

Blood samples were obtained from Korean native cattle and dairy cattle of Holstein species in the slaughter house and methylene blue color tests were performed for the detection of the inherited susceptibity to hemolysis. Glucose-6-phosphate dehydrogenase activities expressed as the optical density obtained by methylene blue color test were the highest as 0.54 in male Korean cattle, 0.62 in female Korean cattle and 0.72 in dairy cattle of Holstein species. Percent hemolysis, packed cell volume and plasma protein contents were measured and compared with relation to the results of methylene blue color test and no correlation was observed in each

In order to investigate the morphological characteristics of dendritic cells in the mammary gland, the appearance on the clear cells(CLs) or ATPase-positive dendritic cells(APDCs) have been observed by the light microscope. CLs were observed in the mammary tissues of the experimental animals, such as mice, rats, guinea pigs, rabbits, cats, dogs, pigs, cows and Korean native goats, and these CLs were confirmed as the ATPase-positive cells of typical dendritic appearance(APDCs). The APDCs were distributed in between the secretory epithelial cells, between the secretory epithelial cells and the myoepithelial cells, the basal area of the secretory epithelial cells, the interalveolar and interlobular connective tissues, and in between the epithelial cells of secretory duct. The APDCs were observed more frequently during the middle period of lactation than the other periods, and were irregularly or uniformly distributed according to the location. The mean difference between the inbred and the noninbred counts in the mice was statistically significant(P0.0001), and the similar result was presented in the rats(P0.0000)

The origin of Jindo dogs has been controversial. It has been informed by words of mouth that they are the offsprings of either the dogs of Sung dynasty (960-1279 A.D.) in China or the Mongolian dogs. Some breeds of dogs in Japan now have almost same appearance as jindo dogs. The authors reviewed the history of Japan and found that Korean hunting dogs were sent to Japan in early 5th century, which was before Sung dynasty of China. Dr. Jae-Keun Ryu of National Institute of Environmental Research has done some work on serums of dogs of both Korea and Japan; the results of his serological and molecular biological studies indicated that Korean dogs were sent to Japan 1,300-2,500 years ago. Jindo dogs appear to be the descendants of the dogs of th Stone Age. They have adapted themselves to the unfavorable environment of Jindo island so that they can maintain pure line-age and wild nature until today. It was concluded that they are Korean native dogs, not the offsprings of the dogs of Sung dynasty or the Mongolian dogs

A porcine parvovirus inactivated vaccine was prepared and inoculated to 7 piglets and also 8 guinea-pigs, and their serum antibodies were titrated. Twenty-two field serum samples of unvaccinated sows were also tested by SN and HI methods. It was observed that SN test was superior over HI test. Therfore, it is suggested that the SN test could well be used in the detection of serum antibody for PPV in vaccinated pigs