Twenty-eight porcine rotavirus were isolated from piglets with diarrhoea in Chonnam Province. According to the age, 41 to 60 day old pigs showed the highest isolation frequency among the post weaning pigs. The characteristics of the field isolates were determined by electronmicroscopy(EM), immunofluorescent assay(FA), and electrophoretic migration patterns of the genome profiles. Some of the isolates showed remarkable haemagglutination activity against rabbit and dog erythrocytes, ranging from 4 to 2848, respectively. At least 3 serotypes of porcine rotavirus were recognized by serum neutralization test using serotype specific rotavirus hyperimmune sera

This study was conducted to isolate etiological agents from the 103 scouring piglets in Kyongnam area and also carried out antimicrobial drug susceptibility test and epidemiogical survey. The incidence of scouring piglet was most prevalent in 81.6% at the age of 2 to 4 weeks after birth, while the rate was less than 10% at the age of 5 to 6 weeks and under 1 week after birth. When comparing the isolation frequency of each etiological agent, enteropathogenic E. coli was most prevalent in 46.6%, thermophilic Campylobacter in 26.2% and Salmonellae was in 8.7% in that order. In the OK serotyping for 117 isolates of enteropathogenic E. coli, type 0141 : K85(20.5%), 0157:K88ac(14.5%), 0138:K81 and 0149:K91 (13.3%) were encountered most frequently. In the biotyping for 27 isolates of thermophilic Campylobacter, most strains of C. jejuni belonged to type I (50.0%) and II (25.0%), and most strains of C. coli belonged to biotype I (78.9%). In the serotyping for 9 strains of Salmonellae, 3 strains were grouped as D, 2 strains as C and 1 strain each was group B and E. The other 2 strains were untypable. The 117 isolates of enteropathogenic E. coli were resistant more than 90% to erythromycin, penicillin, tetracycline and streptomycin, whereas about 90% of the isolates were sensitive to kanamycin and gentamicin. In the case of Salmonellae, all of the isolates were resistant to penicillin, but about 89% of the isolates were sensitive to gentamicin and colistin. All of C. jejuni and C. coli isolates were resistant to cephalothin, but more than 89% of C. jejuni and C. coli were sensitive to kanamycin and gentamicin

This paper deals with the 0 groups of citrate utilizing variants of Escherichia coli (Cit+ E. Coli) isolated from cattle, the production of colicin, hemolysin, K99 antigen, heat stable enterotoxin, and the isolation of plasmid DNA. Among 42 Cit + E. Coli, 12 strains were 020, 9 strains 08, 5 strains 045, 3 strains 0115, 1 strain 064, 1 strain 0139 and remaining strains (11) were untypable. Thirty-nine(81.3%)out of 48 Cit + E. Coli produced colicin and 13(27.0%) produced hemolysin. Of 12 cit + E. coli bearing K99 antigen, 6(50.0%) produced heat stable enterotoxin. In gel electrophoresis for the isolation of plasmid DNA, the number of plasmids varied from 1 to 7 in 10 Cit+ E. Coli. Its molecular weight ranged from 2 to 50 Mdalton, and 50 Mdalton plasmid commonly existed in all strains

To determine the prevalence and the morphological identification of two Eimeria species in fecal examinations, a total of 1092 fecal specimens collected during the period of March to December in 1987 was tested by means of flotation technique. The prevalence of two species was revealed as 1.83% and 0.92% in E. bukidnonensis and E. wyomingensis, respectively. The oocysts of E. bukidnonensis measured in range of 41.3 micro m to 49.4 micro m (mean 45.5+-1.7) x 28.8 micro m to 35.3 micro m (mean 32.2 +-1.2), and E. wyomingensis 36.9 micro m to 44.5 micro m (mean 39.8+-1.7) x 26.5 micro m to 29.9 micro m (mean 28.2+-0.9). The oocysts of E. bukidnonensis appeared darker and more robust than those of E. wyomingensis. The striations in the oocyst wall of E. bukidnonensis were more peculiar than in E. wyomingensis. Among the morphological characteristics, the widths could be reliably taken as a differential point of view for two Eimeria species

To examine the selectivity of verapamil, used in the cardiovascular diseases, on alpha-1 and alpha-2 adrenoceptor-induced pressor responses, effects of verapamil on alpha-adrenoceptor agonist-induced pressor responses were investigated in urethane-anesthetized rabbits, spinal rabbits, rats and pithed rats. To evaluate the effects of verapamil on each pressor response induced by norepinephrine, phenylephrine and clonidine, these agonists were previously injected into an ear vein, and the same procedures were performed 1-2 min after treatment with intravenous verapamil. Intravenous verapamil produced dose-dependent depressor response in rabbits and rats. Pressor responses to intravenous norepinephrine (10 micro g/kg) and phenylphrine (30 micro g/kg) were inhibited by pretreatment with intravenous verapamil in rabbits and no difference was noted between the degree of both inhibitions of the pressor response by verapamil. Pressor responses to intravenous norepinephrine (3 micro g/kg), phenylephrine (20 micro/kg) and clonidine (300 micro g/kg) were inhibited by pretreatment with intravenous verapamil in spinal rabbits. No difference was noted between the inhibition of norepinephrine-induced pressor response and that of phenylephrine-induced pressor response by verapamil. The inhibition of clonidine-induced pressor response by verapamil was more prominent than that of norepinephrine- or phenylephrine-induced pressor response. Pressor responses to intravenous norepinephrine (3 micro g/kg) and phenylephrine (10 micro g/kg) were inhibited by pretreatment with intravenous verapamil in rats and no difference was noted between the degree of both inhibitions of the pressor response by verapamil

The present study was carried out to investigate the estrous behavior and vaginal smear after induction of estus with exogenous hormones in the premature, metestrous and anestrous bitches. In a total of 21 bitches(Mixed breed: 16, Jindo breed: 5), from 10 months to 5 years of age and weighing 8 to 15 kg the change of vaginal smear and the estrous behavior before and after induction of estrus was studied. In the treatment A(They were given the PGF2alpha, estrone, estradiol-17beta, PMSG and HCG) proestrus commenced in 10.16+-1.44(Mean+-SEM) days after treatment. The mean duration of proestrus and estrus was 7.50+-1.44 and 13.50+-3.44 days, respectively. In the treatment B(They were given the PMSG and HCG) proestrus commenced in 5.53+-0.59 days after treatment. The mean duration of proestrus and estrus was 6.60+-0.71 and 14.60+-1.14 days, respectively. All of the 6 bitches in the treatment A showed vulval swelling and vaginal discharge. 14 of the 15 bitches in the treatment B showed vulval swelling and vaginal discharge. However, all of the treatment A and B showed male acceptance. The main change of vaginal smear in proestrus and estrus after induction of estrus was an increase in the proportion of anuclear and superficial cells associated with a decrease in small intermediate and parabasal cells. By the estrous behavior and vaginal smear the estrus was induced in all the premature, metestrous and anestrous bitches

The present study was carried out to investigate C. jejuni and C. coli in chicken. Also were examined the pathogenicity of the isolates in chicks by experimental inoculation. Thermophilic Campylobacter were isolated from 34 (45.9%) of the 74 specimens, and classified as 21.6 % C. jejuni, and 24.3 % C. coli. In the biotyping of 16 strains of C. jejuni isolates, 37.5 % of the strains were grouped as biotype I, 62.5 % as biotype II. In the case of 18 strains of C. coli isolates, 49.9 % of isolates were grouped as biotype I, 55.6 % as biotype II. In oral inoculation with 10** (4)cfu of Capmylobacter isolates into infant chicks (1 to 3 days-old), 17 days-old and 34 days-old chicks, 32.5 % of the chicks developed diarrhea on day 1, 52.5 % on day 3, 70.0 % on day 5, and 27.5% on day 7, and the peak incidence of diarrhea was reached on day 5. The organisms were found to be discharged in feces one day afterwards. C. jejuni and C. coli strains were detected from the feces in 87.5 % of the chicks on day 5.

Appreciable radiation exposures certainly occur in the workers who handle radioiodine in biochemical research, nuclear medicine diagnostics with the development of nuclear industries. But in the case of nuclear accidents, the early medical treatment of radiation injury should be necessary but little was reported in Korea till now. Accordingly, to achieve basic data for protective roles and medical treatment of radiation injury, the present studies were carried out to evaluate the decontamination of radioiodine by the administration of the antithyroid drugs. The administration of sodium iodide and potassium iodide results in rapid excretion of radioiodine and reduction of the whole body retention than the saline-only group. Regarding thyroid protective effects, sodium iodide, potassium iodide results in rapid excretion of radioiodine and reduction of the whole body retention than the saline-only group. Regarding thyroid protective effects, sodium iodide, potassium iodide and saline were effected significant in order. In the control(saline) group, if administered with enough fluids, the whole body retention of radioiodine is reduced temporary shifts. But as far as radioprotective effects are concerned, saline was not more protective than the other groups. In conclusion, in case of nuclear accidents, if being administered sodium iodide and saline as quickly as possible, the radioprotective effects against the radiation hazard might be markedly increased in the internal contamination of radioiodine

The phenotypes of hemoglobin, albumin and transferrin of 302 Jindo dogs in Jindo area were studied by starch gel electrophoresis for hemoglobin and albumin, and by polyacrylamide gradient gel electrophoresis for transferrin. In the hemoglobin phenotypes, three phenotypes, HbAA, HbAB and HbBB, which were controlled by two allelic genes, Hb** (A) and Hb** (B), were observed and their frequencies of appearance were 1.65%, 10.60% and 87.75% respectively. The distribution of gene frequency was calculated as 0.0695 in Hb** (A) and 0.9305 in Hb** (B). In the albumin phenotypes, three phenotypes, Alb FF, Alb FS and Alb SS, which were controlled by two allelic genes, Alb** (F) and Alb** (S) were observed and their frequencies of appearance were 12.59%, 25.56% and 61.85% respectively. The distribution of gene frequency was calculated as 0.2537 in Alb** (F) and 0.7463 in Alb** (S). Analysis of transferrin phenotypes showed 6 different types which were controlled by three allelic genes, Tf** (B), Tf** (C) and Tf** (D), and their frequencies of appearance were 54.04% in TfBB, 17.54% in TfBC, 9.82% in TfBD, 8.07% in TfCC, 7.37% in TfCD and 3.16% in TfDD. The distribution of gene frequency was calculated as 0.6772 in Tf** (B), 0.2053 in Tf** (C) and 0.1175 in Tf** (D)

Serological study and isolation of Brucellae from dairy cattle were carried out in Kyungbook area of Korea during the period from 1984 to 1985. Biotyping of the isolates was done. Of the 11168 cattle in 2479 herds examined, 27 cattle (0.24%) in the 17 herds (o.69%) were serologically positive for brucellosis. Brucella abortus was recovered from 20 (74.1%) of the 27 infected cattle, and all the strains of the isolates belonged to biotype 1. Of the tissues cultured for brucellae, the supramammary lymph nodes had the highest rate (55.6%) of recovery of brucella organisms