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Pharmacokinetics of methylprednisolone acetate after intra-articular administration and its effect on endogenous hydrocortisone and cortisone secretion in horses Texte intégral
2006
Soma, L.R. | Uboh, C.E. | Luo, Y. | Guan, F. | Moate, P.J. | Boston, R.C.
Objective-To determine the pharmacokinetics of methylprednisolone (MP) and develop a pharmacokinetic-pharmacodynamic model of the related changes in plasma concentrations of endogenous hydrocortisone (HYD) and cortisone (COR) following intra-articular administration of methylprednisolone acetate (MPA) in horses. Animals-6 Thoroughbreds. Procedures-In each horse, 200 mg of MPA was injected intrasynovially into a carpal joint, and plasma MP, HYD, and COR concentrations were determined via liquid chromatography-mass spectrometry. Results-A 5-compartment pharmacokinetic-pharmacodynamic model was used to describe the concatenated changes in the plasma concentrations of MP, HYD, and COR and to estimate the instantaneous rate of endogenous HYD production. The median transfer half-life (t(1/2t)) of methylprednisolone from the joint to plasma and elimination half-life (t(1/2e)) from plasma were 1.7 and 19.2 hours, respectively. Maximum plasma concentration of methylprednisolone was 7.26 +/- 3.3 ng/mL at 8 hours, which decreased to 0.11 +/- 0.08 ng/mL at 144 hours after injection. At 3 hours after MPA administration, plasma COR and HYD concentrations were significantly decreased from baseline values (from 2.9 +/- 0.28 ng/mL to 2.10 +/- 1.0 ng/mL and from 61.1 +/- 18.9 ng/mL to 25.7 +/- 12.1 ng/mL, respectively). Conclusions and Clinical Relevance-The sensitivity of the analytic method used allowed complete description of the related kinetics of MP, HYD, and COR following intra-articular administration of MPA. A single intra-articular administration of MPA profoundly affected the secretion of HYD and COR in horses; secretion of endogenous corticosteroids remained suppressed for as long as 240 hours after injection.
Afficher plus [+] Moins [-]Toxicity of lectin extracted from Korean mistletoe (Viscum album coloratum) in chicks and its immunoadjuvant activity on Newcastle disease virus vaccines
2006
Yeo, S.G. (Kyungpook National University, Daegu, Republic of Korea), E-mail: [email protected]
In order to search the availability of the lectin extracted from Korean mistletoe (Viscum album coloratum) as an adjuvant for the avian vaccines, attempts were made to determine toxicity of the lectin in chicks and its immunostimulating activity on the inactivated vaccines against Newcastle disease virus (NDV). For the determination of toxicity, the lectin was injected into the thigh muscle of SPF chicks (Charles River) of 1-week-old and observed hematologically and pathologically.
Afficher plus [+] Moins [-]Sequence analysis of VP2 gene of infectious bursal disease virus field isolate and vaccine strains
2006
Jin, J.D. (Kangwon National University, Chuncheon, Republic of Korea) | Kang, Z.W. (Kangwon National University, Chuncheon, Republic of Korea) | Kim, S.J. (Seoul National University, Seoul, Republic of Korea) | Kwon, H.M. (Kangwon National University, Chuncheon, Republic of Korea) | Hahn, T.W. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: [email protected]
The VP2 full gene of Korean infectious bursal disease virus (IBDV) strain, SH/92, three attenuated vaccine strains, Bur706, Bursine-2 and CEVAC strains, were amplified by reverse transcriptase-polymerase chain reaction and sequenced and compared with published PV2 gene sequences of IBDVs. The VP2 nucleotide sequence similarity between SH/92 and three vaccine stains was 95.6~96.5% whereas the nucleic acid similarity among three vaccine strains was 97.5~98.5%. The amino acid sequence similarity of VP2 of SH/92 compared with three vaccine strains was between 94.4 and 97.6% while the amino acid similarity among three vaccine strains was between 97.4 and 98.4%.
Afficher plus [+] Moins [-]Effects of formalin on haematology and blood chemistry in crucian carp (Carassius auratus)
2006
Im, C.W. (Chungbuk National University, Cheongju, Republic of Korea) | Park, S.C. (Seoul National University, Seoul, Republic of Korea) | Heo, G.J. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: [email protected]
To determine the effects of exposure to formalin on the secondary stress indices, changes in haematology and blood chemistry were monitored in healthy crucian carps (Carassius auratus). Fishes were separately exposed in a concentration range of 125 to 500 ppm formalin for 60 min. After exposure, red blood cell (RBC) count and packed cell volume (PCV) were elevated in the 500 ppm formalin exposed group. However, mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH) were decreased significantly in the 500 ppm formalin exposed group.
Afficher plus [+] Moins [-]A radiographic study of growth plate closure compared with age in the Korean native goat
2006
Choi, H.J. (Chungnam National University, Daejeon, Republic of Korea) | Shin, H.J. (Chungnam National University, Daejeon, Republic of Korea) | Kang, S.K. (Chungnam National University, Daejeon, Republic of Korea) | Lee, H.C. (Gyeongsang National University, Jinju, Republic of Korea) | Cho, J.K. (Chungnam National University, Daejeon, Republic of Korea) | Chang, D.W. (Chungbuk National University, Cheongju, Republic of Korea) | Lee, Y.W. (Chungnam National University, Daejeon, Republic of Korea), E-mail: [email protected] | Jeong, S.M. (Chungnam National University, Daejeon, Republic of Korea) | Park, S.J. (Chungnam National University, Daejeon, Republic of Korea) | Shin, S.T. (Chungnam National University, Daejeon, Republic of Korea)
This study was performed to assess the growth plate closure time with aging in the Korean native goat. Radiographs of proximal and distal epiphysis of humerus, radius, ulna, femur and tibia were obtained at 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 17, 18, 20, 21, 30, 43 and 52 weeks after birth in 30 Korean native goats. The secondary ossification centers were scrutinized and assessed the maturity process on the basis of the criteria (stage 0 to 10). The secondary ossification centers of proximal and distal epiphysis of humerus, radius, femur and tibia and proximal ulna epiphysis appeared immediately after birth.
Afficher plus [+] Moins [-]Application of a soild-phase fluorescence immunoassay to determine ampicillin residues in muscle tissue of olive flounder (Paralichthys olivaceus)
2006
Jung, W.C. (Gyeongsang National University, Jinju, Republic of Korea) | Ha, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) | Chung, H.S. (Gyeongnam Provincial Government, Changwon, Republic of Korea) | Heo, S.H. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, S. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, H.J. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: [email protected]
Parallux∨TM, a solid-phase fluorescence immunoassay (SPFIA) developed for antibiotics residue detection in milk, was applied for analysis of fish muscle. The recommended therapeutic dose of ampicillin (100 mg/kg body weight, withdrawal period 7 days) was orally administered to a group of 25 olive flounders (Paralichthys olivaceus) for consecutive five days. Muscle was sampled after drug treatment 1st, 2nd, 3rd, 4th and 5th day. The concentration of ampicillin in muscle, determined by SPFIA, was compared with that of internal standard (10 ppb as ampicillin).
Afficher plus [+] Moins [-]Effect of ethanol-induced p42/44 MAPkinase activity on IGF system in primary cultured rat hepatocytes
2006
Lee, S.M. (Chonbuk National University, Jeonju, Republic of Korea) | Kim, J.H. (Chonbuk National University, Jeonju, Republic of Korea) | Kang, C.W. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: [email protected]
Ethanol abuse is associated with liver injury, neurotoxicity, modulation of immune responses, and increased risk for cancer, whereas moderate ethanol consumption exerts protective effects against liver injury. However, the underlying signal transduction mechanisms of insulin-like growth factors (IGFs) which play an important regulatory role in various metabolism mechanisms are not well understood. We investigated the effects of ethanol-induced p42/44 activity on IGF-I secretion, IGF-I receptor and IGFBP-1 secretion using radioimmunoassay and western blotting in primary cultured rat hepatocytes.
Afficher plus [+] Moins [-]Effects of Suaeda asparagoides M∧IQ extracts on mice ileal motility
2006
Song, J.C. (Kyungpook National University, Daegu, Republic of Korea) | Park, C.H. (Kyungpook National University, Daegu, Republic of Korea) | Kim, H.T. (Kyungpook National University, Daegu, Republic of Korea) | Endale, Mehari (Kyungpook National University, Daegu, Republic of Korea) | Rhee, M.H. (Kyungpook National University, Daegu, Republic of Korea) | Park, S.C. (Kyungpook National University, Daegu, Republic of Korea) | Kim, K.S. (Kyungpook National University, Daegu, Republic of Korea) | Kim, T.W. (Kyungpook National University, Daegu, Republic of Korea), E-mail: [email protected]
Suaeda (S.) asparagoides M∧IQ, one of the halophyte groups, has been used as a folk remedy for digestive disturbances in Korea. However, its pharmacological activity on gastrointestinal motility has not been reported yet. In this study, the effects of this halophyte extracts with various solvent fractions (ethanol, hexane, chloroform, ethyl acetate, butanol, and water) on mice ileal spontaneous motility was examined. All solvent fractions at the concentration of 100 ㎍/ml showed inhibitory actions on spontaneous motility of ileum with the potency order of water greater-than 70% ethanol greater-than hexane much greater-than chloroform greater-than or equal to butanol greater-than or equal to ethyl acetate, respectively.
Afficher plus [+] Moins [-]Genetic characterization of Shigella flexneri isolated from the diarrheic patients in Seoul region
2006
Seung, H.J. (Seoul Metropolitan City Research Institute of Public Health and Environment, Seoul, Republic of Korea) | Kim, M.S. (Seoul Metropolitan City Research Institute of Public Health and Environment, Seoul, Republic of Korea) | Oh, Y.H. (Seoul Metropolitan City Research Institute of Public Health and Environment, Seoul, Republic of Korea) | Choi, B.H. (Seoul Metropolitan City Research Institute of Public Health and Environment, Seoul, Republic of Korea) | Chae, H.S. (Seoul Metropolitan City Research Institute of Public Health and Environment, Seoul, Republic of Korea) | Chu, J.Q. (Chungnam National University, Daejeon, Republic of Korea) | Jun, M.H. (Chungnam National University, Daejeon, Republic of Korea), E-mail: [email protected]
The shigellae are common etiological agents of bacillary dysentery in humans and primates. During four years from 2002 to 2005, 22 strains of Shigella spp. were isolated from the diarrheic patients in Seoul region. All of them were identified as S. flexneri by biochemical tests and serotyping. The prevalence of serotypes were variable by year, but the major serotypes were 2a and 3a. In an antimicrobial susceptibility test, all of the isolates were resistant to streptomycin and tetracycline, and susceptible to amikacin, kanamycin, cefoxitin, and gentamicin.
Afficher plus [+] Moins [-]The prevalence of pullorum disease-fowl typhoid in grand parent stock and parent stock in Korea, 2003
2006
Kim, A.R. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Kim, J.H. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea), E-mail: [email protected] | Lee, Y.J. (Kyungpook National University, Daegu, Republic of Korea) | Cho, Y.M. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Kwon, J.H. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Kwon, Y.K. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Lee, Y.J. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Choi, J.G. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Joh, S.J. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Kim, M.C. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Lee, E.K. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Kim, C.S. (Ministry of Agriculture and Forestry, Gwacheon, Republic of Korea) | Yang, H.G. (Ministry of Agriculture and Forestry, Gwacheon, Republic of Korea) | Kwag, S.I. (Ministry of Agriculture and Forestry, Gwacheon, Republic of Korea) | Sung, H.W. (Kangwon National University, Chuncheon, Republic of Korea) | Mo, I.P. (Chungbuk National University, Cheongju, Republic of Korea)
Serum samples of 30 chickens per flock from 6 grand parent stock (GPS) farms and 70 parent stock (PS) farms were collected for seroprevalent study of pullorum disease-fowl typhoid (PD-FT) infection by serum plate agglutination test (SPA). The incidence of PD-FT infection in GPS flocks and PS flocks were 0% and 15.7%, respectively. Especially PS flocks infected with PD-FT showed age dependent patterns that 22.2% of flocks between 20 to 30 weeks of age and 38.9% of flocks between 30 to 40 weeks of age were positive.
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