It is often difficult to predict how synergistic effects work and how dieback may be expected to spread through an area over time. On the other hand concepts and methods for compensation of acid deposition, melioration and restauration of acidified forest areas represent a major management problem. The following examples show how GIS may be used to find answers to these questions.

Work was carried out to investigate the effect of pollution on the needle cytokinin content of mature Picea abies (L.) Karst individuals. Needles were collected from trees growing at two sites, one polluted and relatively non-polluted, in central Slovenia. After ethanolic extraction and purification, the needle cytokinin extracts were separed by reverse-phase HPLC. Cytokinin content was analysed using four direct ELISA techniques specific for Z-, DHZ- and iP-type cytokinins and the O-glucoside conjugate of Z. Results showed a trend towards the accumulation of cytokinins in needles from trees subject to high levels of atmospheric pollution, particulary with respect to ribotide derivatives. No difference was found in the (OG)Z content between polluted and non-polluted samples. The results are discussed with respect to the origins of the observed accumulation.

Types of ectomycorrhizae on Norway spruce were determined in soil cores from two differently polluted forest research plots from the emission zone of Sostanj Thermal Power Plant (TPP). The two plots are comparable regarding site characteristics, but are differently polluted by the emmissions from the TPP. During the vegetations season 1993, 21 soil cores were taken from each plot. In these all fine roots and ectomycorrhizal roots tips were counted and the percentages of different types were calculated. Additionally, soil mycorrhizae from underneath fungal fruit bodies were taken in order to characterize new types of ectomycorrhizae. Classical anatomical methods and molecular tools were applied for identification. 17 types of ectomycorrhizae were determined on 28443 root tips in soil cores from the heavily polluted plot (Zavodnje). The predominant types were Paxillus involutus, Xerocomus badius, Piceirhiza parallela and Piceirhiza inflata. On the less polluted plot (Mislinja) 24 different types were determined on 38502 root tips in equal volume and number of soils cores. The most frequent types were Hydnum rufescens, Amphinema byssoides, Piceirhiza oleiferans, Cenococcum geophilum and Lactarius lignyotus. Mycobioindication of forest site pollution through selective sensitive (here Hydnum rufescens), in comparison to unsensitive (here Paxillus involutus), fungal species in ectomycorrhizae is discussed.

This paper deals with presenting a broad range of possibilities, which can be used with computer equipment by deriving, interpreting and processing forest data. Different rates of generalized data from the forest inventory can be used as a criterion in preparing different solutions. So this data strongly influences decision making processes. The paper also presents cost analysis and multi-criteria evaluation methods.

The paper summarizes the main results of reach on heavy metal detoxification in ectomycorrhizas of Rhizopogon roseolus, Paxillus involutus, Cenococcum geophilum and Pisolithus arrhizus formed on Pinus sylvestris roots collected from heavily polluted areas in Poland. The data were obtained by methods including electron microscopy (TEM, SEM) accompanied by EELS and EDAX, cytochemical stainings and capillary electrophoresis. All of the species described in the paper are fungi highly resistant to toxic elements. As detoxification in mycorrhizas is dependent on both partners, the phenomenon was studied in fungi and in Pinus sylvestris roots. The strongest element-filtering effect was observed in R. roseolus / P. sylvestris symbiosis. This mycorrhiza type was characterized by the accumulation of heavy metals in the fungal mantle, and a gradual decrease of these elements was observed along the Hartig net towards the inside of the root. Potentially toxic elements were localized intercellularly in crystals deposited on the mantle and hyphal surface and in pigmented layer of the cell wall of the extramatrical hyphae, and the hyphae of the outer mantle layer. They were also detected intracellularly within electron-opaque depositions in the vacuoles. Much lower levels of toxic elements were detected in plant cells. The most common place for heavy metal accumulation was the phenolic material of the cortical cells.

Cytokinins were analysed by a combined HPLC-ELISA method in needles of Norway spruce seedlings. The seedlings were grown in vitro on sterile or nonsterile soil substrates from two differently polluted forest research plots. Difference were predominantly found in the isopentenyladenine-type of cytokinins. These were elevated in seedlings, grown on polluted soils in comparison to those, grown on soils from the unpolluted plot. A possible explanation might be in the change of the metabolism in the roots due to pollution stress (when grown on sterilized substrates) or in change of the mycorrhizosphere organisms (when grown on nonsterile soil substrates). The model system using Norway spruce seedlings as tester organisms for soil pollution is discussed.

Plant hormones play a major role in the development and control of a wide range of plant physiological processes. Here we examine the ways in which they may influence the response of plants to air pollutants both indirectly, for example through the control of stomatal behaviour by ABA and directly, through their reactions with oxidising pollutants, which can lead to the formation of further damaging molecular species.

The evaluation of data on the forest tree condition is weak point in forest decline monitoring as it enables only a very rough estimation of the state of forest health. The paper analyses the interpretation of the forest tree defoliation data in order to estimate their relevance and applicability. Quality control results of defoliation assessment in Slovenia are presented and the state of defoliation with its changes in the year 1987, 1991, 1993, 1994 on the 16 km grid.

The condition of silver fir (Abies alba Mill.) trees was monitored 1988-1994 by measuring electrical resistance of living tissues at breast height. Slime cells in bark and traumatic resin canals in wood were investigated as possible symptoms of silver-fir decline.

Seven ectomycorrhizae on oak (Quercus robur L.) from a Slovenian forest stand are described, five of them for the first time in detail. Four of these species could be identified (Lactarius chrysorrheus (Fr.) Fr., L. serifluus DC. ex Fr., Xerocomus subtomentosus (L. ex Fr.) Quel., X. armeniacus (Quel.) Quel., one unidentified species was given a preliminary name ("Quercirhiza squamosa"). Two identified species, already comprehensively characterized on other tree species, are presented shortly (Laccaria amethystina (Bolt ex Hooper) Murr., Cenococcum geophilum Fr.). Whereas some of the encountered species show considerable host specifity with oak (Lactarius chrysorrheus, L. serifluus and, in a lesser degree Xerocomus armeniacus), other species have a broader host range and can even grow with conifers (Xerocomus subtomentosus, Laccaria amethystina, Cenococcum geophilum). A key for all species was established. A possible use of ectomycorrhizal species as bioindicators is discussed.