International audience | In situ denitrification walls and biofilters made of wood chips are being implemented as innovative technologies for the removal of nitrates in tile drainage water from farms to reduce pollution of surface waters and the hypoxia problem in the Gulf of Mexico. Although fairly effective in removing nitrates, not much is known about the effectiveness of the biofilters in removal of herbicides, pesticides, and antibiotics in the drainage water. Using weathered wood chips obtained from an in situ denitrification wall, four common pollutants tested sorbed strongly to wood chips in the following order: enrofloxacin > monensin A > atrazine > sulfamethazine. Of the four chemicals tested, enrofloxacin was found to desorb the least by water extraction. The apparent hysteresis index for atrazine was found to be lower than that for enrofloxacin and sulfamethazine indicating greater sorption–desorption hysteresis for atrazine than enrofloxacin and sulfamethazine. Consecutive steps of water desorption and organic solvent extraction indicated that more than 65% of the sorbed atrazine, 70% of sulfamethazine, 90% of enrofloxacin, and 80% of monensin A were retained in wood chips. Results of this study showed that wood chip denitrification walls or biofilters have an added benefit in retaining herbicides and antibiotics and therefore can act as a barrier to reduce pollution of surface water and groundwater.

International audience | We present here the effects of ambient ozone (O3)-induced decline in carbon availability, accelerated foliar senescence, and a decrease in aboveground biomass accumulation in the Aleppo pine (Pinus halepensis Mill.). Aleppo pine seedlings were continuously exposed in open-top chambers for 39 months to three different types of O3 treatments, which are as follows: charcoal-filtered air, nonfiltered air (NFA), and nonfiltered air supplemented with 40 ppb O3 (NFA+). Stable carbon isotope discrimination (Δ) and derived time-integrated ci/ca ratios were reduced after an accumulated ozone exposure over a threshold of 40 ppb (AOT40) value from April to September of around 20,000 ppb·h. An AOT40 of above 67,000 ppb·h induced reductions in ribulose-1, 5-biphosphate carboxylase/oxygenase activity, aboveground C and needle N and K concentrations, the C/N ratio, Ca concentrations in twigs under 3 mm, and the aerial biomass, as well as increases in needle P concentrations and phosphoenolpyruvate carboxylase (PEPC) activity and the N and K concentrations in twigs under 3 mm. Macronutrients losses, the limitations placed on carbon uptake, and increases in catabolic processes may be the causes of carbon gain diminution in leaves which was reflected as a reduction in aboveground biomass at tree level. Stimulation of PEPC activity, the consequent decreased Δ, and compensation processes in nutrient distribution may increase O3 tolerance and might be interpreted as part of Aleppo pine acclimation response to O3.

International audience | Riparian wetlands are subject to nitrogen enrichment from upgradient agricultural and urban land uses and also from flooding by nitrogen-enriched surface waters. The effects of this N enrichment on wetland soil biogeochemistry may be mediated by both the presence of plants and the presence of redox-active compounds, specifically iron oxides in the soil. Despite the extensive research on wetland N cycling, the relative importance of these two factors on nitrogen is poorly known, especially for forested wetlands. This study evaluates the responses of the N and the Fe cycles to N enrichment in a riparian forested wetland, contrasting vegetated field plots with plots where the vegetation was removed to test the role of plants. Furthermore, in vitro anaerobic incubations of the experimental soils were performed to track Fe chemical changes over time under anoxic or flooded conditions. Wetland soils treated with N in form of urea, as expected, had significantly higher amounts inorganic nitrogen. In the soils where vegetation was also removed, in addition to inorganic nitrogen pool, increase in organic nitrogen pool was also observed. The results demonstrate the role of vegetation in limiting the effects excess urea has on different soil nitrogen pools. Results from anaerobic incubation of the experimental soils demonstrated the effects of N enrichment on the wetland Fe cycle. The effects of excess nitrogen and the role of vegetation on the Fe cycle in riparian wetland soil became more evident during anaerobic incubation experiments. At the end of the field experiment, Fe concentrations in the soils under the treatments were not significantly different from the control soils at the 5% confidence level. However, during the anaerobic incubation experiment of soils collected at the end of the experiment from these plots, the N-enriched soils and the unvegetated soils maintained significantly elevated concentrations of reducible Fe(III) for the initial 2-week period of incubation, and the soils collected from the plots with both the treatments had the highest Fe(III) concentrations. After 20 days of incubation, however, the Fe (III) concentrations decreased to the similar concentrations in all the incubated soils. The study clarifies the roles vegetation play in mediating the effects of N enrichment and also demonstrates that N enrichment does affect wetland redox cycle, which has strong implications on ecosystem services such as water quality improvement.

International audience | Composting may enhance bioremediation of PAH-contaminated soils by providing organic substrates that stimulate the growth of potential microbial degraders. However, the influence of added organic matter (OM) together with the microbial activities on the dissipation of PAHs has not yet been fully assessed. Materials and methods An in-vessel composting-bioremediation experiment of a contaminated soil amended with fresh wastes was carried out. Four different experimental conditions were tested in triplicate during 60 days using laboratory-scale reactors: treatment S (100%soil),W(100%wastes), SW(soil/ wastemixture), and SWB(soil/wastemixture with inoculation of degrading microorganisms). Results and discussion A dry mass loss of 35±5% was observed in treatments with organic wastes during composting in all the treatments except treatment S. The dissipation of the 16 USEPA-listed PAHs was largely enhanced from no significant change to 50.5±14.8% (for SW)/63.7±10.0% (for SWB).More obvious dissipation was observed when fresh wastes were added at the beginning of composting to the contaminated soil, without significant difference between the inoculated and non-inoculated treatments. Phospholipid fatty acid (PLFA) profiling showed that fungi and G-bacteria dominated at the beginning of experiment and were probably involved in PAH dissipation. Subsequently, greater relative abundances of G+bacteria were observed as PAH dissipation slowed down. Conclusions The results suggest that improving the composting process with optimal organic compositions may be a feasible remediation strategy in PAH-contaminated soils through stimulation of active microbial populations.

International audience | Mining activities generate spoils and effluents with extremely high metal concentrations of heavy metals that might have adverse effects on ecosystems and human health. Therefore, information on soil and plant metal concentrations is needed to assess the severity of the pollution and develop a strategy for soil reclamation such as phytoremediation. Here, we studied soils and vegetation in three heavily contaminated sites with potential toxic metals and metalloids (Zn, Pb, Cd, As, TI) in the mining district of Les Malines in the Languedoc region (southern France). Extremely high concentrations were found at different places such as the Les Aviniéres tailing basins (up to 160,000 mg kg-1 Zn, 90,000 mg kg-1 Pb, 9,700 mg kg-1 of As and 245 mg kg-1 of Tl) near a former furnace. Metal contamination extended several kilometres away from the mine sites probably because of the transport of toxic mining residues by wind and water. Spontaneous vegetation growing on the three mine sites was highly diversified and included 116 plant species. The vegetation cover consisted of species also found in non-contaminated soils, some of which have been shown to be metaltolerant ecotypes (Festuca arvernensis, Koeleria vallesiana and Armeria arenaria) and several Zn, Cd and Tl hyperaccumulators such as Anthyllis vulneraria, Thlaspi caerulescens, Iberis intermedia and Silene latifolia. This latter species was highlighted as a new thallium hyperaccumulator, accumulating nearly 1,500 mg kg-1. These species represent a patrimonial interest for their potential use for the phytoremediation of toxic metal-polluted areas.

The present work deals with the characterization of selected heavy metals (Ti, V, Cr, Mn, Cu, Mo, Pb, Ni, Cd and As) in PM10 collected in 2008 in three urban areas of Cantabria (Northern Spain) which are influenced by industrial activities. Ternary plots were used to identify the main tracers at the studied sites. Pollutant roses of these tracers were used as tools which give information about the location of the most important emission sources due to the characteristic wind patterns in Cantabria. Pollutant roses showed a significant contribution of the local industry to trace metal levels at the selected urban sites. | The authors would like to thank the financial support of CTM 2006-00317 project (MEC) and the Government of Cantabria (“Actions for improving the air quality and its diagnosis in Cantabria”) for their funding. We would like to express our gratitude to the Regional Environmental Ministry of Cantabria Government who kindly provided the dataset from ALM sampling point.

The bacterial communities in the anoxic layer of a heavily polluted microbial mat and their growth on hydrocarbons under sulfate-reducing conditions were investigated. Microbial communities were dominated by members of Alphaproteobacteria (27% of the total rRNA), Planctomycetes (21.1%) and sulfate-reducing bacteria (SRB: 17.5%). 16S rRNA cloning revealed sequences beloning to the same bacterial groups with SRB affiliated to the genera Desulfobulbus, Desulfocapsa, Desulfomicrobium, Desulfobacterium and Desulfosarcina/Desulfococcus. The derived enrichment cultures on crude oil, hexadecane and toluene were dominated by SRB. While most SRB sequences of the toluene and hexadecane cultures were related to the sequence of Desulfotignum toluolica, the crude oil enrichment showed a more diverse bacterial community with sequences from the genera Desulfotignum, Desulfobacter, Desulfatibacillus, Desulfosalina, and Desulfococcus. We conclude that the anoxic layer of the studied mats contains a diverse community of anaerobic bacteria, dominated by SRB, some of which are able to grow on hydrocarbons.

Coral community structure is often governed by a suite of processes that are becoming increasingly influenced by land-use changes and related terrestrial discharges. We studied sites along a watershed gradient to examine both the physical environment and the associated biological communities. Transplanted corals showed no differences in growth rates and mortality along the watershed gradient. However, coral cover, coral richness, and coral colony density increased with increasing distance from the mouth of the bay. There was a negative relationship between coral cover and mean suspended solids concentration. Negative relationships were also found between terrigenous sedimentation rates and the richness of adult and juvenile corals. These results have major implications not only for Pacific islands but for all countries with reef systems downstream of rivers. Land development very often leads to increases in river runoff and suspended solids concentrations that reduce coral cover and coral diversity on adjacent reefs.