The survival of inoculated in a cold-smoked sausages Listeria monocytogenes wild strains was studied. The sausages were prepared with and without starter cultures. The survival limits of L. monocytogenes and lactic acid bacteria (LAB) were determined as colony forming units per gram (cfu gE-1) depending on water activity (aw) and pH on 0, 1st, 3rd, 5th, 7th, 14th and 21st days of maturation. The decreasing water activity conditioned by moisture (weight) loss during ripening and pH decrease ensured negative polynomial growth rate of inoculated L. monocytogenes - 0.27 lg (cfu gE-1) each day of ripening time, and - 0.65 lg (cfu gE-1) on the first 7 days of maturation. A significant Pearson’s correlation (p is less than 0.01) was established between decreased values of L. monocytogenes count, aw, salt concentration and LAB growth in sausages during the ripening period of 21 days. The main parameters, maintained negative exponential growth rate of L. monocytogenes in cold smoked sausages, are aw value decrease and LAB (starter culture), which stopped L. monocytogenes growth at the beginning of cold-smoked sausage maturation. If fermentation process went technically and hygienically correctly, the fermented (cold-smoked) sausages could be one of the safest meat products, because in real practice a low level contamination has been seen. The remaining count of L. monocytogenes in cold-smoked sausage depends on the possible initial contamination level and could exceed the European Union regulation value 2.0 lg (cfu gE-1) for ready-to-eat products when contamination at first is more than lg 5.0.

In this work the combinations of commercial lactic acid bacteria starters and acetic acid bacteria strain were used for production of fructans in substrate, both with and without sucrose additive, and studied their potential in maintaining technological properties of yoghurt and fermented milk. The objective of this study was to assess the effect of fructans producing starter cultures on milk coagulation technique, the amount of secreted fructans and viscosity of fermented milk samples. An amount of fructans synthesized by starter cultures and Gluconobacter sp. B35, pH and viscosity of samples were measured using appropriate standards and analytical methods. Results showed that the addition of acetic acid bacteria did not influence the pH dynamics of fermented milk samples. Increasing sucrose concentration in samples significantly influences fructans production potential. The application of such technology in fermented dairy product production would have potential from microbiological exopolysaccharides increasing position with the aim to promote functionality of dairy foods and to substitute commercial stabilisers etc. The addition of acetic acid bacteria in milk showed negative impact on viscosity of the evaluated samples. The viscosity was liquid in all analyzed samples with acetic acid bacteria, the addition of sucrose helped to make the consistency of yoghurt and fermented milk more liquid. We concluded that the structure of synthesized fructans could not help to improve the textural properties of fermented dairy products. From this point of view, the studied acetic acid bacteria strain should have the potential as prebiotic.