To determine microbial populations and their viability, methods assessing colonies formed and turbidity are generally used. However, these methods require a long incubation time. Thus, a rapid colorimetric microbial viability assay based on the reduction of the tetrazolium salt WST-8 via 2-methyl-1,4-naphthoquinone as an electron mediator was developed. The applicability of the assay for susceptibility testing of various bacteria to antibiotics, screening of antimicrobial substances, determination of water-soluble vitamins, and differentiation of Gram-positive and -negative bacteria was investigated. Good agreement was observed between results with the WST-8 colorimetric method and those obtained using conventional methods. The present assay method was superior to the conventional methods with respect to the overall rapidity, accuracy and convenience.

A burgeoning population, pressing development needs and increasing household consumption are rapidly accelerating water use in direct and indirect ways. Increasingly, regions around the world face growing pressure on sustainable use of their water resources especially in arid and semi-arid regions, such as Northern China. The aim of this research is to obtain an overview of the cumulative water requirement for direct (domestic) water use and indirect water use for the basic food consumption of the households in the Inner Mongolia Autonomous Region (IMAR), in order to reduce the pressure on grassland of Western China by encouraging sustainable water consumption. For indirect water use, we use VWC (virtual water content) analysis theory to analyze the total consumption package of 15 basic food types that were identified and quantified based on the household survey in 2011. In this survey, domestic water consumption data and food consumption data were collected from 209 representative households with spatial variation across three sub-regions (including meadow steppe in Hulun Buir, typical steppe in Xilin Gol, and semi-desert steppe in Ordos) and temporal variation from 1995 to 2010. The results show that the total amounts of food consumption per capita in three sub-regions all show an increasing trend, especially in Hulun Buir and Ordos. Compared to the direct water consumption, the indirect water consumption behind food production made up a major portion of total water consumption, which is affected (1) geographic locations (grassland types); (2) economic development levels and (3) grassland use policy measures. From 1995 to 2010, indirect water consumption displays a decreasing trend in Xilin Gol and Ordos due to the decrease of meat consumption and increase of fruit and vegetable consumption. When considering the amount of land per household, the grassland in Ordos still faces the great threat of high water consumption pressure. Such water consumption may affect water conservation services and productivity of grassland. Therefore, changing diet behavior and reducing the population can be considered options for sustainable use of water.

Strategies for reducing food waste and developing sustainable diets require information about the impacts of consumption behavior and waste generation on climatic, water, and land resources. We quantified the carbon, water, and ecological footprints of 17,110 family members of Chinese households, covering 1935 types of foods, by combining survey data with available life-cycle assessment data sets. We also summarized the patterns of both food consumption and waste generation and analyzed the factors influencing the observed trends. The average person wasted (consumed) 16 (415) kg of food at home annually, equivalent to 40 (1080) kg CO2e, 18 (673) m3, and 173 (4956) gm2 for the carbon, water and ecological footprints, respectively. The generation of food waste was highly correlated with consumption for various food groups. For example, vegetables, rice, and wheat were consumed the most and accounted for the most waste. In addition to the three plant-derived food groups, pork and aquatic products also contributed greatly to embedded footprints. The data obtained in this study could be used for assessing national food security or the carrying capacity of resources.

A sensitive liquid–chromatography–electrospray tandem mass spectrometry multiclass method for determination of 45 veterinary compounds belonging to 9 different antibiotic groups, including aminoglicosides (4), β-lactams (13), diaminopyrimidines (1), fluoroquinolones (10), lincosamides (1), macrolides (5), pleuromutilins (1), sulfonamides (6) and tetracyclines (4), in water from breeding animal watering supply system has been developed. Isolation of the analytes was carried out by solid phase extraction with heptafluorobutyric acid as an ion-pair agent on the Strata-X reversed phase cartridges. All analytes were determined simultaneously in one single run on a C18 column with gradient elution and short analysis time (13min). Method was validated, average relative recoveries were in the range of 84.3–109.3% with satisfactory precision are repeatability for all compounds are in the range of 4.7–12.2%, within-laboratory reproducibility are in the range of 4.4–13.5% for. The limit of quantitation (LOQ) of the method was in the range of 0.02–10μgL−1, depending of analyte. The applicability of the method was tested by determining antimicrobial compounds in real water samples collected from water supply systems in breeding animal farms. The average antibiotics concentration in real water samples were, respectively, in the range of 0.14–1670μgL−1.

This study summarizes the concept of the food-energy-water security nexus (FEW nexus). The aim is to create awareness about the importance of the nexus and to enable stakeholders to consider interconnections between the sectors in their work. The FEW nexus is discussed in the context of Africa south of the Sahara (SSA)—using Malawi and Mozambique as case studies. Even though analyzing food, energy, and water security issues simultaneously is critical given the interconnections, summarizing interventions with the FEW nexus approach in Malawi and Mozambique, we found that there are only a limited number of interventions in place. Additionally, this study reviews macro- and microeconomic models that are able to analyze the FEW nexus. On the macrolevel, especially general equilibrium models are discussed, because they show trade-offs and synergies of nexus interventions at all economic levels. These models can help guide policymakers’ understanding of nexus effects ex ante and convince them to think beyond their respective political departments. On the microlevel, the impact of nexus interventions can be assessed with qualitative and quantitative approaches. There are specific challenges for nexus interventions when it comes to aggregation and planning of the targeting. A secondary data analysis of nexus interventions shows that existing data is not sufficient to conduct research specifically related to the FEW nexus. The results of this study will help research programs to reflect the key questions required to enhance adoption of FEW technologies and inform policymakers as they formulate policies that will exploit the strong synergies of food security, energy, and water investments. | Non-PR | IFPRI1; A.2 Sustainable Natural Resource Policies; A Ensuring Sustainable food production; CRP2; Land Resource Management for Poverty Reduction | EPTD; PIM | CGIAR Research Program on Policies, Institutions, and Markets (PIM)

Growth rates of individual freshwater shrimp of the species Atya lanipes and Xiphocaris elongata were measured in a second‐order stream in the Luquillo Experimental Forest in Puerto Rico over 10 years (1997–2007). Shrimp living at lower altitudes in warmer water and wider stream channels with more algal and detrital foods were predicted to grow and reproduce more rapidly. Shrimp were marked and recaptured periodically in pools located at three altitudes to determine whether temperature affected growth rates among individual A. lanipes and X. elongata. Mean annual water temperatures ranged from 20 to 24 °C with the uppermost pool being cooler than the lower pools. Mean annual growth rates for Atya and Xiphocaris were 0.27 and 0.1 mm carapace length, respectively, for all three populations. Differences in growth were partially influenced by how each species obtains its food. Atya is a filter feeder and scraper and has continuous access to suspended organic particles and biofilms. The slower growth rate for Xiphocaris elongata is most likely a result of the wider range in quality and accessibility of food resources. Differences in pool morphology and depths probably affected differences in food availability. Increased leaf litter retention in the deeper upper and lower pools probably increased shrimp growth rates, while washout of leaf litter from the relatively shallow, elongate mid‐altitude pool decreased Atya lanipes growth rates. These long‐lived, slow‐growing shrimp species transform a wide range of organic materials into their biomass. Because of the slow growth rates of these detritivores shrimp, tropical storms, hurricanes, droughts or other disturbances could have persistent, long‐term impacts on detrital processing and on the populations of their predators.

Lateral flow immunoassays (LFIAs) are advantageous over conventional detection methods in terms of their simplicity and rapidity. These assays have been reported using various types of labels but colloidal gold nanoparticles are still the preferred choice as a label because of their easy synthesis, visual detection and stability. Bacterial contamination of food and drinking water is a major threat and hindrance towards ensuring food and water safety. Enterobacteriaceae family members are mainly transmitted by the consumption of contaminated water and food and implicated in various food or water borne infections. The LFIAs have been popularly used for detection of bacterial cells in different matrices. Therefore, this review intends to provide an analysis of the gold nanoparticle based lateral flow assays developed for detecting enterobacteriaceae family members in food and water samples. The review includes detailed data and discusses the factors that influence the performance of LFIAs and their shortcomings.

This article uses the WaterWorld Policy Support System, coupled with a global database for commodity flows, to examine the impacts of AR4 SRES climate change scenarios on Africa's drylands and the commodity flows that originate from them. It shows that changes to precipitation and, to a lesser extent, temperature in Africa's drylands can significantly affect the potential to supply water-for-food locally and internationally. By comparing the geographical distribution of climate change with the supply chain–connected distribution of climate change, it shows how food-water impacts of climate change may affect local dryland populations but also those dependent on these flows from afar.

This work studied the effect of composition of powders and water content on mixture quality (MQ) of three binary powder mixtures with good (salt/paprika or salt/sugar) or poor mixing (salt/oregano) behaviour. The mixing behaviour was assessed using coefficient of variation. Results showed that mixture composition did not influence the MQ of sugar/salt and paprika/salt within 20–80% salt content range but it did influence the MQ of oregano/salt with a progressive dis-improvement in MQ with higher oregano content and also for low concentrations of 1% salt. Water content did have an effect on mixing behaviour. When paprika with high water activity (aw) was mixed with salt, the time required to reach good MQ was longer because of the increased cohesiveness and when oregano with high aw was mixed with salt it displayed improved MQ because salt particles were able to stick onto the larger oregano particles and reduced segregation.

The prospect of climate change has revived both fears of food insecurity and its corollary, market opportunities for agricultural production. In Australia, with its long history of state-sponsored agricultural development, there is renewed interest in the agricultural development of tropical and sub-tropical northern regions. Climate projections suggest that there will be less water available to the main irrigation systems of the eastern central and southern regions of Australia, while net rainfall could be sustained or even increase in the northern areas. Hence, there could be more intensive use of northern agricultural areas, with the relocation of some production of economically important commodities such as vegetables, rice and cotton. The problem is that the expansion of cropping in northern Australia has been constrained by agronomic and economic considerations. The present paper examines the economics, at both farm and regional level, of relocating some cotton production from the east-central irrigation areas to the north where there is an existing irrigation scheme together with some industry and individual interest in such relocation. Integrated modelling and expert knowledge are used to examine this example of prospective climate change adaptation. Farm-level simulations show that without adaptation, overall gross margins will decrease under a combination of climate change and reduction in water availability. A dynamic regional Computable General Equilibrium model is used to explore two scenarios of relocating cotton production from south east Queensland, to sugar-dominated areas in northern Queensland. Overall, an increase in real economic output and real income was realized when some cotton production was relocated to sugar cane fallow land/new land. There were, however, large negative effects on regional economies where cotton production displaced sugar cane. It is concluded that even excluding the agronomic uncertainties, which are not examined here, there is unlikely to be significant market-driven relocation of cotton production.