Inactivation of renin in a mixed mitochondrial-lysosomal fraction of post-partum uterus [in vitro, hog renal renin, Triton X-100, ATP, alfa-ketoglutarate, iodoacetate, phenylmethylsulphonylfluoride]
1979
Joergensen, J. (Copenhagen Univ. (Denmark). Inst. of Experimental Medicine)
The marked renin inactivation seen during in vitro incubation of postpartum uterine slices which mimics the in vivo condition, is not accompanied by a similar general proteolysis. The inactivating mechanism is so far non-specific with respect to organ or species as added hog renal renin is inactivated at a similar rate as endogenous renin. Endogenous alkaline phosphatase is not significantly inactivated and added alkaline phosphatase is completely stable. A marked inactivation of endogenous renin also takes place during incubation of a mixed mitochondrial-lysosomal suspension prepared from post-partum uterus. The process is more pronounced at pH 7.4 than at 6.8. Freezing and thawing and addition of Triton X-100 prior to incubation inhibits the inactivation. ATP and alpha-ketoglutarate slightly stimulates the process while CoA and chloroquine have no effect. Both iodoacetate and phenymethylsuphonylfluoride inhibit the inactivation, suggesting that more than one enzyme is involved in the inactivation.
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