Isolation and translation of hordein messenger RNA from wild type and mutant endosperms in barley [hordein precursor, high lysine mutant, prolamin]
1978
Brandt, A. | Ingversen, J. (Atomenergikommissionens Forsoegsanlaeg, Risoe (Denmark))
Messenger RNA has been isolated from developing barley endosperms by sucrose gradient sedimentation. Sepharose 4B gel filtration and preparative gel electrophoresis. Hordein messenger RNA was found to be a major constituent of the total messenger RNA population of the endosperm cell. Polyadenylated hordein messenger RNA sedimented at 11S in sucrose gradients and electrophoretic analysis reveals the presence of at least three RNA species with apparent molecular weights of 0.45, 0.36 and 0.30 megadaltons. The 11S messenger RNA was translated in vitro into hordein precursor polypeptides which are 2-4 kilodaltons larger than the native hordein polypeptides. The endosperm cell of mutant No. 1508 contained twice as much RNA as the wild type endosperm cell but he same amount of polyadenylated 11S RNA. The template activity of the latter was 10 per cent of that found for the 11S hordein messenger RNA from the wild type and was limited to translation into one hordein precursor polypeptide
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