Effects of inhibitors on the activity of cAMP phosphodiesterase in the mouse oocytes
1986
Jung, M.W. | Cho, W.K. (Seoul National Univ., Seoul (Korea R.). Dept. of Zoology)
The purpose of the present experiment was to make certain the existence of cAMP phosphodiesterase (PDE) in mouse oocytes and confirm its possible role on meiotic resumption. The results showed two types of cAMP in the oocytes with different Michaelis constants (Km) with specific maximum (Vmax); The Km and Vmax of one of two types of PDE were estimated at 0.14 +- 0.01 micro M and 0.42 +- 2.0 micro M and 2.2 +- 0.5 fmol cAMP hydrolyzed/ oocyte/ minute
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