Cell biological studies on growth and development. Effect of Ca2+ and polyamine of Beta-glucan synthetase activity in carrot root protoplast
1987
Lee, S.H. | Kang, Y.H. | Pyo, B.S. (Yonsei Univ., Seoul (Korea R.). Dept. of Biology) | Cho, Y.D. (Yonsei Univ., Seoul (Korea R.). Dept. of Biochemistry) | Kim, M.W. (Yonsei Univ., Wonju (Korea R.). Dept. of Biology) | Lee, J.S. (Ewha Womans Univ., Seoul (Korea R.). Dept. of Biology)
The effect of polyamine, Ca2+ and calmodulin on GS (Beta-glucan synthetase) activity was studied in Daucus carota root. The Ca2+ is shown to have no effect on the GS activity whereas the GS II activity increased in response to increase in concentration of the Ca2+. When the protoplasts are cultured, for 4 days, the GS II activity increases as a function of time and reaches a maximum after 3 days at a time when the network of cellulose microfibrils is known to be synthesized. The effect of the Ca2+ and 1mM spermine on the GS II activity turns out to be synergistic, especially more synergistic at lower concentration of the Ca2+. The GS II activity seems to be enhanced by the Ca2+. The GS II activity in the protoplast treated by the calcium channel blocker, verapamil, turns out to be lower than that of the control. Cumulative results suggest that the Ca2+ stimulates the cell wall regeneration via enhancement of the GS II activity responsible for synthesizing the cell wall component through synergistic effect with spermine
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