Tissue culture of Pittosporum resiniferum Hemsl. (Petroleum nut tree)
1990
Zamora, C.V. (Philippines Univ., Diliman, Quezon City (Philippines). Inst. of Biology)
Explants (blade with midrib or without midrib and leaf tip, 6 x 5 mm) from very young leaf of Pittosporium resiniferum produced more significant results about 90-100% compared to the 20-80% of explants from young leaf. However, leaf blade with midrib (from very young leaf) produced culture growth earlier than any of the explants tried; and plantlets were formed after subcultures of 5-8 weeks old growing explants in 1 l of Revised Murashige and Skoog's [RMS] supplemented with 10 mg Benzyl adenine (BA), 0.25 naphthalene acetic acid (NAA), and 0.25 dichlorophenoxy acetic acid (2,4-D). The explants (blade with midrib) from very young leaf became enlarged, fleshy and green after 21 days culture and later produced an excellent and remarkable cabbage - like callus growth. After 30 days culture, these calli became green and friable creamy-greenish-yellow and roots (whitish structures) were observed. These culture growth characteristics were also observed after 40 days. Whereas, these morphological callus characteristics were observed in explants from blade and leaf tip of very young leaf, only after 40 days culture. The best culture medium is 1 l of RMS agar medium supplemented with 10 mg BA, 0.25 NAA, and 0.25 2,4-D at pH 5.7 exposed to light laboratory condition. Other treatments were tried in the tissue culture of its plant parts, such as: agar media with 1 l RMS supplemented with different concentration, of growth regulators such as BA, Kinetin (Ki), NAA, indole-butyric acid (IBA), 2,4-D, and gibberellic acid (GA3) and with or without coconut water as adjuvant from mature fruits (CW) and young fruits (CW) from 20, 25, 30, 35, 50, and 100 ml. In these treatments, some did not form any callus growth.
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