Influence of low temperature on amino acid pool in the eggs of the silkworm, Bombyx mori
1994
Furusawa, T. (Kyoto Univ. of Industrial Arts and Textile Fibers (Japan). Faculty of Textile Science) | Ishihara, R.
A series of experiments was conducted to determine whether the amino acid pool in the eggs undergoing diapause in the silkworm, Bombyx mori was influenced by low temperatures (5 and 0 degrees C). Amounts of alanine (Ala), proline (Pro) and glutamate (Glu) were different among the eggs kept at different temperatures. In the eggs continuously kept at 25 degrees C after oviposition, the amounts of glu and Pro increased until day 160 after oviposition and reached a level of 20 micro-moles/g eggs. Ala accumulated at a level of 55 micro-moles up to day 30 after oviposition, and then the level increased gradually. Chilling (5 or 0 degrees C) of the eggs induced a decline in the Ala content during diapause maintenance. In the eggs that had completed the diapause termination as were transferred to 5 degrees C, the decrease continued but was limited in the diapausing eggs transferred to 0 degrees C. On the other hand, the Glu content decreased around the diapause termination in the eggs transferred to 5 degrees C, but continued to increase in the diapausing eggs kept at 0 degrees C. In contrast, the level of Pro accumulation was higher in the eggs kept at 5 degrees C than at 0 degrees C. These results suggest that a low temperature of 0 degrees C but not 5 degrees C suppresses the metabolic pathway of Glu to Pro in Bombyx eggs
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