Isolation of protoplasts from suspension culture and subsequent shoot regeneration in sugarcane [Saccharum]
1996
Matsuoka, M. (Japan International Research Center for Agricultural Sciences, Ishigaki, Okinawa. Okinwa Branch) | Terauchi, T. | Kobayashi, M. | Shoda, M. | Nakano, H.
Cell suspension cultures with an ability to regenerate plants were initiated from 10 sugarcane clones tested. Cultures were initiated and maintained in modified N6 liquid medium containing 2 mg/l 2,4-D, 500 mg/l casein hydrolysate and 3% sucrose. Protoplasts isolated from these suspensions were embedded in 1.2% agarose (modified KM8P medium) and cultured in modified KM8P medium with the addition of nurse culture cells from suspension culture. After 5 to 8 weeks of culture, calluses were obtained in 5 clones. Calluses derived from protoplasts could not form any organs in the following culture on R2 regeneration medium. Only, protoplast-derived calluses of NiF4 (cultivar) cultured for 2 weeks on PR4 medium regenerated green shoots on R9 medium. These shoots could not develop to plant, and died in the following culture
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