Development of phosphine reagents for fluorometric determination of lipid hydroperoxides [in foodstaff and biological materials]
1995
Akasaka, K. (Tohoku Univ., Sendai (Japan). Faculty of Agriculture)
Phosphine reagents wee designed and synthesized as new type of fluorescence reagents to determine lipid hydroperoxides in foodstuff and biological materials. All phosphine reagents prepared had no fluorescence but their oxides, which were produced by the reaction of phosphines with hydroperoxides, had strong fluorescence. Among the phosphine reagents prepared, diphenyl-1-pyrenylphosphine (DPPP) had the most suitable properties as a fluorescent reagent. This reagent was successfully applied to the determination of lipid hydroperoxides by both batch method and HPLC-post column method
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