Callus induction and organogenesis in pechay (Brassica chinensis L.) cv. black behi
1993
Lapitan, V.C. | Tandang, C.A. | Patena, L.F. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding)
This is the first report on callus induction and subsequent organogenesis in pechay (Brassica chinensis L. cv Black Behi). Calli initiated from embryos using MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 mg/L 6-benzylaminopurine (BAP) and 2 percent sucrose formed green masses which regenerated shoots and roots when transferred to the regeneration medium. Shoot regeneration (20 percent) occurred on MS medium containing combinations of 0.5-1.0 mg/L zeatin and 0.1-1.0 mg/L napthaleneacetic acid (NAA) and 3 percent sucrose. On the other hand, root formation (73 percent) was obtained on medium with higher concentrations of zeatin (1.5-2.0 mg/L) in combination with 0.1 mg/L NAA. This breakthrough is of great potential as an alternative means of propagation and as an in vitro system for genetic transformation in pechay
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