Movement and distribution of potato leafroll virus antigen in resistant potato genotypes
1999
Ahmad, W. (Ayub Agricultural Research Inst., Faisalabad (Pakistan)) | Thomas, P.E.
Twelve potato clones with variable degrees of resistance to potato leafroll virus (PLRV) were co-infected with potato virus X (PVX) or potato virus Y (PVY) before or after inoculations with PLRV. In some PLRV resistant clones, PVX and PVY facilitated the movement, and also increased the concentration of PLRV antigen. PLRV could not be rub-transmitted in mixed infections with the sap-transmissible viruses, PVX and PVY. PLRV did not observably affect the movement and concentration of PVX and PVY in resistant potato clones. PLRV also did not affect the movement of PVX into non-inoculated leaf tissue of cultivars Saco and USDA seedling 41956, nor did it cause systemic movement of PVX in hypersensitive Gomphrena globosa. PLRV was detected in infected plants by enzyme-linked immunosorbent assay (ELISA) and tissue blotting on nitrocellulose membranes, using polyclonal antibodies. Tissue blotting proved to be a more sensitive method, especially for detection of low titer antigens from plant tissue. These studies indicate that an essential function of PLRV inhibited in resistant plants may be at least partially complemented by active function of PVX or PVY.
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Эту запись предоставил National Agricultural Research Centre
