Potato virus detection by tuber testing

Starovic, M.S.

Potato virus detection by tuber testing

1998

Starovic, M.S.

Nectrotic strain of Y virus (Y(N)) and potato leaf roll virus (PLRV) most frequent potato viruses, on the most diffused variety Desire, in Serbia (Yugoslavia). For the detection of Y virus and PLRV from tubers the following modifications of enzime-linked immunosorbent assay (ELISA) are applied: "cocktail", DAS, "amplified" and MUP, as well as bioassay. The highest sensibility was manifested by the "amplified" test, and slightly lower by DAS and MUP ELISA. In Y virus detection "cocktail" manifested higher sensibility in comparison to DAS and bioassay. Complete tuber infection of plants secondary infected by Y virus and PLRV was detected by ELISA. Investigating the possibilities of Y virus and PLRV detection from tubers, the highest values of absorption and the biggest number of infected tubers was proved by testing on samples from tuber buds of plants primary infected by Y virus, and from heel-end of tubers infected by PLRV. The occurrence of Y virus in 18-62% and PLRV in 40% of tubers (germ) of primary infected plants was proved by bioassay. For rapid detection of virus occurrence in potato tubers the application of ELISA and of its modifications give satisfying results. It is proposed that for Y virus detection samples should be taken from tuber rose-end, and testing should be carried out by "cocktail" ELISA, while for PLRV sampling is carried from hill-end of tuber and testing by DAS ELISA. Due to the complexity of the "amplified" ELISA, it should be used only when precise answer about tuber infection is needed.

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