Expression of myrosinase-associated protein in Escherichia coli
1999
Anggraeni, G.A.A.R. | Meryandini, A. (Institut Pertanian Bogor (Indonesia)) | Andreasson, E.
The myrosinase-glucosinolate system is one of the plant defense systems, which exists mainly in the Brassicaceae family. Myrosinase hydrolyzes glucosinolates into several products that some of them are toxic for certain herbivores. Some types of myrosinases form a high molecular complexes with other non-myrosinase proteins. One of these non-myrosinase proteins is Myrosinase-Associated Protein (MyAP), which is a 40 kD glycosilated protein with at least one intramolecular disulfide bridge. MyAP exists both in complex with myrosinase and in a free form. Based on its sequence similarity with other proteins with known activities, MyAP has been proposed to have an esterase activity. One of the cDNA of induced MyAP (iMyAP9) has been expressed in E. coli in the inclusion body fraction.
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