Propagation of black mangrove Bruguiera gymnorrhiza Lamk. by tissue culture and hypocotyl cutting techniques
1997
Pipat Patanaponpaiboon | Kullanart Obsuwan | Patchra Limpanavech (Chulalongkorn Univ., Bangkok (Thailand). Faculty of Science. Dept. of Botany)
Study on improvement of hypocotyl cutting techniques of Bruguiera gymnorrhiza Lamk. was conducted by dividing hypocotyl into 2 pieces, apical half and basal half with root stimulating agents; auxins, IBA and NAA. Both IBA and NAA had different effects on root number and root length formed on the cuttings. The apical half produced more roots than the basal one. In the apical cuttings both IBA and NAA 1000 mg/l were considered to be the best concentration for root inducing. Shoot tips, leaf dises, receptacle and hypocotyls from black mangrove (Bruguiera gymnorrhiza) were cultured on Murashige & Skoog media and WPM, supplemented with various form of auxin; (IBA, 2, 4-D) and cytokinins (BAP). In vitro culture of various parts of B. gymnorrhiza contamination was a serious problem. Explants used derived from plant tissues cultured in vitro produced a lot of browning substance which had an inhibitory effect on cell division and growth. This experiment thus was carried out to examine the disinfestation techniques and the application of chemicals and methods to decrease excretion of brownning substance. The success of in vitro induction of shoot formation from hypocotyl explant provided a method to solve contamination problem and these shoots produced were aseptic and were able to subculture further. The suitable medium for shoot induction from hypocotyl section culture was concluded, however the medium for root induction needed further study.
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