In vitro screening of sunflower for resistance to Sclerotinia sclerotiorum (Lib.) de Bary

Vasic, D.; Alibert, G.; Skoric, D.

In vitro screening of sunflower for resistance to Sclerotinia sclerotiorum (Lib.) de Bary

1999

Vasic, D. (Institute of Field and Vegetable Crops, Novi Sad (Yugoslavia)) | Alibert, G. | Skoric, D.

Protoplasts isolated from hypocotyls and leaves of two genotypes of the cultivated sunflower and from stems and leaves of Helianthus maximiliani were cultured in the presence of different concentrations of oxalic acid, putative toxin of Sclerotinia sclerotiorum. Their viability was determined by staining with fluorescein diacetate during five days. Statistical analysis showed that there was significant difference in reaction to oxalic acid between the genotypes as well as between different organs of the same genotype. Protoplasts of Helianthus maximiliani were found to be more resistant to stress with oxalic acid than protoplasts isolated from the two genotypes of the cultivated sunflower which is in accordance with the results obtained in field conditions. Differential reaction of protoplasts isolated from different plant organs to oxalic acid indicates that this method could be used for screening for resistance to different forms of Sclerotinia.

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