Detection of Lawsonia intracellularis in Hungarian swine herds by polymerase chain reaction
1998
Biksi,I. | Kacskovics,I. | Mandoki,M. | Ivan,J. | Horvath-Papp,I. | Makay,G.
Pooled faecal samples and/or intestinal contents from pig carcasses were collected from 11 Hungarian pig farms and subjected to DNA extraction/purification and subsequent poymerase chain reaction (PCR) in order to detect Lawsonia intracellularis.Specific PCR positivity was detected in 6 individual and 22 pooled samples out of 46, originating from 8 herds.The PCR products of collected faecal samples hybridized in Southern blot hybridization with the DNA of the type strains L.inracellularis NCTC 12657.This appears to be the first confirmed detection of L.inracellularis by PCR in Hungary.
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