Selection and identification of Promicromonospora sp. KH-28 producing Chitinase and antifungal antibiotic

Han, K.H.; Kim, S.D. . Department of Applied Microbiology, College of Natural Resources

Selection and identification of Promicromonospora sp. KH-28 producing Chitinase and antifungal antibiotic

1999

Han, K.H. | Kim, S.D. (Yeungnam University, Kyongsan (Korea Republic). Department of Applied Microbiology, College of Natural Resources)

A multifunctional antagonistic bacterium, producing both antifungal antibiotic and chitnase that could be used as biocontrol agents against fungal plant pathogens was isolated from the plant-diease suppressive soil. The isolate was identified as Promicromonospora sp. KH-28 from various morphological, biochemical and physiological tests. The antifungal antibiotic produced by Promicromonospora sp. KH-28 was soluble in n-butanol, methanol, toluene, n-hexane, ethanol but insoluble in H2O, acetone, chloroform, ethylacetate and etylether. It inhibited the growth of fungal plant pathogens of Fusarium solani, F. oxysporum, Alternaria mali and Phytophthora capsici. The antagonistic Promicromonospora sp. KH-28 produced optimally the antifungal antibiotic when it was cultivated at pH 7, 30 degrees centigrade for 5 days.

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