How to characterize meiotic functions in plants? [forward genetics, reverse genetics] | [Comment caractériser les fonctions de la méiose chez les plantes? [génétique directe, génétique inverse]]
2001
Mercier, R. ((Institut National de la Recherche Agronomique, Versailles (France). Centre de Versailles Grignon, Station de Génétique et d'Amélioration des Plantes)) | Grelon, M. | Vezon, D. | Horlow, C. | Pelletier, G.
Our understanding of plant meiosis is rapidly increasing thanks to the model Arabidopsis thaliana. Here we present the results of a screening for meiotic mutants carried out with a library containing 30 719 T-DNA insertion lines. An average of one mutant per 1000 lines was recovered. Several phenotypic classes could be distinguished and are presented. In parallel, 39 proteins known to be involved in meiosis in non-plant organisms were chosen and a search was performed for homologue sequences in the completed Arabidopsis thaliana genome. Approximately 30/ of the meiotic related sequences showed similarities with one or several Arabidopsis putative genes. The relevance of forward versus reverse genetics in order to characterize meiotic functions is discussed
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