Sequence and Characterization of the Genomic Clone of the FVFD16 and FVFD30 Gene Isolated from Flammulina velutipes
2000
Kim Dool-Yi Forestry Research Institute, Seoul (Korea Republic) Tomo-Nori Azuma Hokkaido Forest Products Research Institute, Nishikagura 1-10, Asahikawa 017-01, Japan
We isolated genomic clone of FVFD16 and FVFD30 gene specifically expressed during fruit body formation of Falmmulina velutipes [(Curt:Fr.) Sing] and determinated the sequences. The FVFD16 gene is including two introns in open reading frame, and FVFD30 gene is including four introns. The introns were matched GT/AG rule. The FVFD16 and FVFD30 genes contained CAAT box with similarity arrange and TATA box. CT-rich region was presented before the transcription start point. FVFD30 gene is investigated that expected the most activity of CCACC arrange. The result of FVFD16 gene analysis showed 80% homology by cDNA clone that is gene family. From the results of genomic southern blot analysis, we presumed more than two copy number gene family of FVFD16 and FVFD30 gene.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Korea Agricultural Science Digital Library