Sequence and Characterization of the Genomic Clone of the FVFD16 and FVFD30 Gene Isolated from Flammulina velutipes
2000
Kim Dool-Yi Forestry Research Institute, Seoul (Korea Republic) Tomo-Nori Azuma Hokkaido Forest Products Research Institute, Nishikagura 1-10, Asahikawa 017-01, Japan
We isolated genomic clone of FVFD16 and FVFD30 gene specifically expressed during fruit body formation of Falmmulina velutipes [(Curt:Fr.) Sing] and determinated the sequences. The FVFD16 gene is including two introns in open reading frame, and FVFD30 gene is including four introns. The introns were matched GT/AG rule. The FVFD16 and FVFD30 genes contained CAAT box with similarity arrange and TATA box. CT-rich region was presented before the transcription start point. FVFD30 gene is investigated that expected the most activity of CCACC arrange. The result of FVFD16 gene analysis showed 80% homology by cDNA clone that is gene family. From the results of genomic southern blot analysis, we presumed more than two copy number gene family of FVFD16 and FVFD30 gene.
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