Dot ELISA as a rapid method for serological diagnosis of canine brucellosis

Radojicic, S. . Department of Infectious Diseases; Lako, B.; Djuricic, B.; Valcic, M.

Dot ELISA as a rapid method for serological diagnosis of canine brucellosis

2001

Radojicic, S. (Faculty of Veterinary Medicine, Belgrade (Yugoslavia). Department of Infectious Diseases) | Lako, B. | Djuricic, B. | Valcic, M.

In this work, we describe a dot-enzyme linked immunosorbent assay with two different antigens of Brucella canis. The antigens, soluble sonic and outer membrane proteins were adsorbed on to a solid nitrocellulose surface. The test was applied to 40 field serum samples previously analysed by the rapid slide agglutination test and the 2-mercaptoethanol tube agglutination test. Sera were obtained from clinically suspected dogs of different age, sex and breed. Sera were also obtained from four clinically healthy animals and used as a negative control. One sample originated from a female with a high antibody titer and positive isolation of Brucella canis. Out of the 44 examined sera, 19 had agglutination titers 1/50. A positive reaction to proteins of the soluble sonic extract, was obtained with 10 sera and with outer membrane proteins with 7 sera. All positive sera in the dot-ELISA had agglutination titers 1/100. This study shows that proteins of soluble sonic extract of Brucella canis in dot-ELISA are reliable and highly specific for rapid diagnosis of brucellosis in dogs.

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