Establishment of virus-free taro (Colocasia esulenta cv. Fenghuayunaitou) by meristem-tip culture combined with thermotherapy
2002
Li, Y. | Xu, C. | Chen, J. (Zhejiang Univ., Hangzhou (China). Coll. of Life Science)
Virus-free taro (Colocasia esculenta) was obtained by elimination of dasheen mosaic virus (DsMV) through thermotherapy and meristem-tip culture. Shoot tips as explants of field taro (cv. Fenghuayunaitou) were cultured on MS basal salts medium supplied with 2.0 mg l-1 benzylamiopurinne (BA) and tips were proliferated and proliferated for production of new buds. When the rootless seedlings transferred on MS basal salts medium, plantlets with roots were obtained within 20 days. For detection of DsMV from tube-seedlings, a molecular probe of the gnomic RNA of DsMV HZ isolate (DsMV-HZ) was constructed by RT-PCR, for the 31 end partial sequence and used for dot blotting hybridization with 32P probe. Analysis of nucleotides and deduced amino acid sequence between DsMV-HZ isolate with documented references presented 80-95% similarity among the coat protein region and 31 end untranslated region. About 10% seedlings obtained by meristem-tip culture were identified as DsMV- free seedlings in this experiment.
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