Detection of Pseudomonas aeruginosa with a Lanel-free Immunosensor from Various Cold Storage Foods
2003
Kim, N.S. | Park, I.S. | Kim, D.K. (Korea Food Research Institute, Songnam, Republic of Korea)
The aim of this study is to develop a label-free immunosensor for microbial detection and to evaluate its applicability to Pseudomonas aeruginosa detection in various food samples. The antibodies used were a polyclonal antiserum from rabbit and a monoclonal antibody raised against the flagella of P. aeruginosa. Antibody immobilization was done by a thiolated antibody chemisorption on to one gold electrode of a piezoelectric quartz crystal with a thiol-cleavable, heterolbifunctional cross-linker, sulfosuccinimidyl 6-[3-(2-pyridyldithio) propionamido] hexanoate. To the Stomacher-treated samples from various raw and processed foods under cold storage, comprising sirloin, cod and pettitoes, spiking and enrichment culture were done to prepare the model samples, followed by the measurements of the frequency shifts after sample injections
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