Purification and Assay of Extracellular Autolysin from Moraxella sp. CK-1
2003
Oh, Y.S.Lee, C.H. | Ha, M.S. | Yoon, M.Y. (Hanyang University, Seoul, Korea Republic)
Moraxella sp. CK-1 is known to inhibits the growth of Anabaena cylindrica , a cyanobacterium. It has been documented that the ability of this growth inhinition of Anabaena cylindrica was attributed to extracellular aurolysin from Moraxella sp. CK-1. However, it remains to be elucidated identification and characterization of autolysin have yet been elucidated. In this study, we tried to purify and identify autolysin secreted from Moraxella sp. CK-1. Cells were grown in a complex liquid medium and culture supernatants were collected, followed by ammonium sulfate fractionation. Fractions were further separated with anion exchange column, Mono-Q, in FPLC system and analyzed by SDS/PAGE. The fraction containing high autolysin activity showed a single distinct protein peak in anion column and molecular mass of about 17kDa in SDS/PAGE. N-proteases, including extracellular serine protease, Dichelobacter nodosus.
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