Morphology, chromosome counts and genomic analysis of abnormal haploid offspring of clariids (Clarias macrocephalus)
1997
Krit Mongkonpunya | Chokechai Senawong | Wikrom Rungsin | Amara Thongpan | Saowaluk Thinchant | Tiersch, Terrence (Kasetsart Univ., Bangkok (Thailand). Faculty of Science. Dept. General Science)
Morphological abnormality (haploid syndrome) of fry was obtained from crosses between ultraviolet-irradiated (0.3 J/square cm) eggs of Clarias gariepinus and fresh sperm of C. macrocephalus. The abnormal fry had a short, curved tail and an enlarged cavity between the yolk sac and anterior part of the body. Chromosome counts and analysis of genome size indicated that the abnormal fry were haploid, and amplification by polymerase chain reaction of genomic DNA using primers targeting a portion of the gene encoding the immunoglobulin heavy chain confirmed inheritance of the paternal genome. These findings will aid in distinguishing diploid from haploid androgenetic offspring.
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