Potassium thiocyanate extract vaccine of Pasteurella multocida and its comparison with conventional bacterin vaccine in mice and goats

Sharma, R.K.; Rahman, H.

Potassium thiocyanate extract vaccine of Pasteurella multocida and its comparison with conventional bacterin vaccine in mice and goats

2003

Sharma, R.K. | Rahman, H. (Assam Agricultural University, Guwahati (India))

Crude potassium thiocyanate (KSCN) extract of Pasteurella multocida serotype A was prepared and used as vaccine in poats and mice. KSCN extract consisted of 7 detectable proteins with molecular weight (MW) ranging form 29 - 205 KDa. The vaccine conferred 100% protection in mice against homologous (Pasteurella multocida A) challenge, but it could protect only 66.67% mice against heterologous(Pasteurella haemolytica A2) challenge. The bacterin vaccine could protect 66.67 and 50.00% of mice against homologous and heterologous challenges, respectively. The serum antibody titre expressed in log 2 scale reached the peak at the third week in goats vaccinated with KSCN (7.33) and bacterin preparation (5.16) respectively. Difference in antibody titre developed by the 2 vaccines was statistically significant (P0.05)

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