Antioxidant Action of Reaction Mixtures of Gums Hydrolysates and Urea Derivatives
2004
Kim, S.W. (University of Osaka, Osaka, Japan) | Park, G.G. (Kyungwon University, Seoungnam, Republic of Korea), E-mail: [email protected]
The purified β-mannanase hydrolyzed various gums to mannose, β-1,4-mannobiose, Gal³Man₄, and D.P 7 of galactosyl mannooligosaccharide, and isolated from the enzymatic hydrolysate for 24 hrs reaction by activated carbon column chromatography and Sephadex G-25 column chromatography. For the elucidate of antioxidant action of β-1,4-mannobiose, Gal³Man₄ and DP 7 of galactosyl mannooligosaccharide and urea derivatives, coloration, reducing power, antioxidant activity and DPPH test were accomplished. The coloration was high at reaction mixture of β-1,4-mannobiose, Gal³Man₄, D.P 7 and urea.
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