Isolation and Characterization of an Alkaline Cellulase Produced by Alkalophilic Bacillus sp. HSH-810
Kim, J.Y.;Hong, J.H.(Inje University, Gimhae, Republic of Korea)E-mail:fdsnhhon@inje.ac.kr | Hur, S.H.(Dong-eui institute of technology, Busan, Republic of Korea)
A bacterium producing alkaline cellulase was isolated from soil, leaf mold and compost, and was identified as alkalophilic Bacillus sp. HSH-810 by morphological, cultural and biochemical determination. The optimum cul-ture condition of Bacillus sp. HSH-810 for the growth and alkaline cellulase production was 30℃ and pH 10.0. The maximum alkaline cellulase production was obtained when 1.0％(w/v) CMC, 0.5％(w/v) peptone, 0.02％(w/v) CaCl₂ and 0.02(w/v) CoCl₂ were used as carbon source, nitrogen source and mineral source, respectively. The optimum pH and temperature of the enzyme activity were pH 10.5 and 50℃, respectively.
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