Two tombusviruses isolated from lisianthus (Eustoma grandiflorum (Raf.) Shinn.) with necrotic stunt
2006
Fujinaga, M.(Nagano-ken. Vegetable and Ornamental Crops Experiment Station (Japan)) | Morikawa, T. | Doi, M. | Yoneyama, C. | Ibrahim, M. | Ogiso, H. | Miyamoto, K. | Miyasaka, M. | Ohki, T. | Kameya Iwaki, M. | Natsuaki, T.
From diseased leaves of Lisianthus plants [Eustoma grandiflorum (Raf.) Shinn.] with necrotic stunt symptoms Nagano and Shizuoka prefectures in Japan, we isolated two viruses with a diameter of ca. 30 nm and tentatively named them Nag-4 and Shiz-1, respectively. Both viruses reproduced necrotic spots on healthy lisianthus plants after mechanical inoculation. Based on virion morphology, double-stranded RNA analysis, molecular mass of the coat protein (CP) and serological tests, the viruses were closely related to species in the genus Tombusvirus. In a comparison of the amino acid sequence of the CP genes, Shiz-1 had high identity with that of Tomato bushy stunt virus-nipplefruit strain (TBSV-Nf), whereas Nag-4 shared less than 87% identity with sequences reported for tombusviruses, suggesting that the virus might be a new species in the genus Tombusvirus. This is the first report of tombusvirus disease on lisianthus in the world.
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